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Research Conducted at Amgen Has Provided New Information about Immunoglobulins (Resolution of heterogeneous charged antibody aggregates via...

July 23, 2014



Research Conducted at Amgen Has Provided New Information about Immunoglobulins (Resolution of heterogeneous charged antibody aggregates via multimodal chromatography: A comparison to conventional approaches)

By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Immunology have been published. According to news reporting from West Greenwich, Rhode Island, by NewsRx journalists, research stated, "Clearance of aggregates during protein purification is increasingly paramount as protein aggregates represent one of the major impurities in biopharmaceutical products. Aggregates, especially dimer species, represent a significant challenge for purification processing since aggregate separation coupled with high purity protein recovery can be difficult to accomplish."

The news correspondents obtained a quote from the research from Amgen, "Biochemical characterization of the aggregate species from the hydrophobic interaction and cation exchange chromatography elution peaks revealed two different charged populations, i.e. heterogeneous charged aggregates, which led to further challenges for chromatographic removal. This paper compares multimodal versus conventional cation exchange or hydrophobic chromatography methodologies to remove heterogeneous aggregates. A full, mixed level factorial design of experiment strategy together with high throughput experimentation was employed to rapidly evaluate chromatographic parameters such as pH, conductivity, and loading. A variety of operating conditions were identified for the multimodal chromatography step, which lead to effective removal of two different charged populations of aggregate species."

According to the news reporters, the research concluded: "This multimodal chromatography step was incorporated into a monoclonal antibody purification process and successfully implemented at commercial manufacturing scale."

For more information on this research see: Resolution of heterogeneous charged antibody aggregates via multimodal chromatography: A comparison to conventional approaches. Biotechnology Progress, 2014;30(3):636-645. Biotechnology Progress can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (American Chemical Society - www.acs.org; Biotechnology Progress - www.pubs.acs.org/journal/bipret)

Our news journalists report that additional information may be obtained by contacting R.A. Chmielowski, Amgen Inc, West Greenwich, RI 02817, United States. Additional authors for this research include S. Meissner, D. Roush, T.O. Linden, E. Glowacki, J. Konietzko and J. Nti-Gyabaah (see also Immunology).

Keywords for this news article include: Antibodies, Immunology, Rhode Island, United States, West Greenwich, Blood Proteins, Immunoglobulins, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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