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Reports Outline Food Science Study Results from K.T. Kongstad et al (High-Resolution Screening Combined with HPLC-HRMS-SPE-NMR for Identification of...

July 24, 2014



Reports Outline Food Science Study Results from K.T. Kongstad et al (High-Resolution Screening Combined with HPLC-HRMS-SPE-NMR for Identification of Fungal Plasma Membrane H+-ATPase Inhibitors from Plants)

By a News Reporter-Staff News Editor at Agriculture Week -- Investigators discuss new findings in Food Science. According to news originating from Copenhagen, Denmark, by VerticalNews correspondents, research stated, "Crude extracts of 33 plant species were assessed for fungal plasma membrane (PM) H+-ATPase inhibition. This led to identification of 18 extracts showing more than 95% inhibition at a concentration of 7.5 mg/mL and/or a concentration-dependent activity profile."

Our news journalists obtained a quote from the research, "These extracts were selected for semi-high-resolution fungal PM H+-ATPase inhibition screening, and, on the basis of these results, Haplocoelum foliolosum (Hiern) Bullock and Sauvagesia erecta L. were selected for investigation by high-resolution fungal PM H+-ATPase inhibition screening. Structural analysis performed by high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy (HPLC-HRMS-SPE-NMR) led to identification of chebulagic acid (1) and tellimagrandin II (2) from H. foliolosum. Preparative-scale isolation of the two metabolites allowed determination of IC50 values for PM H+-ATPase, and growth inhibition of Saccharomyces cerevisiae and Candida albicans."

According to the news editors, the research concluded: "Chebulagic acid and tellimagrandin II are both potent inhibitors of the PM H+-ATPase with inhibitory effect on the growth of S. cerevisiae."

For more information on this research see: High-Resolution Screening Combined with HPLC-HRMS-SPE-NMR for Identification of Fungal Plasma Membrane H+-ATPase Inhibitors from Plants. Journal of Agricultural and Food Chemistry, 2014;62(24):5595-5602. Journal of Agricultural and Food Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Journal of Agricultural and Food Chemistry - www.pubs.acs.org/journal/jafcau)

The news correspondents report that additional information may be obtained from K.T. Kongstad, PCOVERY, DK-2200 Copenhagen, Denmark. Additional authors for this research include S.G. Wubshet, A. Johannesen, L. Kjellerup, A.M.L. Winther, A.K. Jager and D. Staerk.

Keywords for this news article include: Europe, ATPase, Denmark, Copenhagen, Food Science, Enzymes and Coenzymes

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Agriculture Week


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