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Recent Studies from Yale University Add New Data to Biomedicine and Biomedical Engineering [Detection of cerebral NAD(+) by in vivo H-1 NMR...

July 23, 2014



Recent Studies from Yale University Add New Data to Biomedicine and Biomedical Engineering [Detection of cerebral NAD(+) by in vivo H-1 NMR spectroscopy]

By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Biotechnology. According to news reporting originating in New Haven, Connecticut, by NewsRx journalists, research stated, "Nicotinamide adenine dinucleotide (NAD+) plays a central role in cellular metabolism both as a coenzyme for electron-transfer enzymes as well as a substrate for a wide range of metabolic pathways. In the current study NAD(+) was detected on rat brain in vivo at 11.7T by 3D localized H-1 MRS of the NAD(+) nicotinamide protons in the 8.7-9.5 ppm spectral region."

The news reporters obtained a quote from the research from Yale University, "Avoiding water perturbation was critical to the detection of NAD(+) as strong, possibly indirect cross-relaxation between NAD+ and water would lead to a several-fold reduction of the NAD(+) intensity in the presence of water suppression. Water perturbation was minimized through the use of localization by adiabatic spin-echo refocusing (LASER) in combination with frequency-selective excitation. The NAD(+) concentration in the rat cerebral cortex was determined at 296 +/- 28 mu M, which is in good agreement with recently published 31P NMR-based results as well as results from brain extracts in vitro (355 +/- 34 mu M). The T-1 relaxation time constants of the NAD(+) nicotinamide protons as measured by inversion recovery were 280 +/- 65 and 1136 +/- 122 ms in the absence and presence of water inversion, respectively. This confirms the strong interaction between NAD(+) nicotinamide and water protons as observed during water suppression. The T-2 relaxation time constants of the NAD(+) nicotinamide protons were determined at 60 +/- 13 ms after confounding effects of scalar coupling evolution were taken into account. The simplicity of the MR sequence together with the robustness of NAD(+) signal detection and quantification makes the presented method a convenient choice for studies on NAD(+) metabolism and function."

According to the news reporters, the research concluded: "As the method does not critically rely on magnetic field homogeneity and spectral resolution it should find immediate applications in rodents and humans even at lower magnetic fields."

For more information on this research see: Detection of cerebral NAD(+) by in vivo H-1 NMR spectroscopy. NMR in Biomedicine, 2014;27(7):802-809. NMR in Biomedicine can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; NMR in Biomedicine - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1099-1492)

Our news correspondents report that additional information may be obtained by contacting R.A. de Graaf, Yale University, Sch Med, Dept. of Psychiat, Magnet Resonance Res Center, New Haven, CT 06520, United States (see also Biotechnology).

Keywords for this news article include: Biotechnology, New Haven, Connecticut, United States, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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