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Recent Findings from Genentech, Inc. Provides New Insights into Immunoglobulins (Effect of ambient light on monoclonal antibody product quality...

July 23, 2014



Recent Findings from Genentech, Inc. Provides New Insights into Immunoglobulins (Effect of ambient light on monoclonal antibody product quality during small-scale mammalian cell culture process in clear glass bioreactors)

By a News Reporter-Staff News Editor at Biotech Week -- Fresh data on Immunology are presented in a new report. According to news originating from San Francisco, California, by NewsRx correspondents, research stated, "During a small-scale cell culture process producing a monoclonal antibody, a larger than expected difference was observed in the charge variants profile of the harvested cell culture fluid (HCCF) between the 2 L and larger scales (e.g., 400 L and 12 kL). Small-scale studies performed at the 2 L scale consistently showed an increase in acidic species when compared with the material made at larger scale."

Our news journalists obtained a quote from the research from Genentech, Inc., "Since the 2 L bioreactors were made of clear transparent glass while the larger scale reactors are made of stainless steel, the effect of ambient laboratory light on cell culture process in 2 L bioreactors as well as handling the HCCF was carefully evaluated. Photoreactions in the 2 L glass bioreactors including light mediated increase in acidic variants in HCCF and formulation buffers were identified and carefully analyzed. While the acidic variants comprised of a mixture of sialylated, reduced disulfide, crosslinked (nonreducible), glycated, and deamidated forms, an increase in the nonreducible forms, deamidation and Met oxidation was predominantly observed under light stress. The monoclonal antibody produced in glass bioreactors that were protected from light behaved similar to the one produced in the larger scale."

According to the news editors, the research concluded: "Our data clearly indicate that care should be taken when glass bioreactors are used in cell culture studies during monoclonal antibody production."

For more information on this research see: Effect of ambient light on monoclonal antibody product quality during small-scale mammalian cell culture process in clear glass bioreactors. Biotechnology Progress, 2014;30(3):562-570. Biotechnology Progress can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (American Chemical Society - www.acs.org; Biotechnology Progress - www.pubs.acs.org/journal/bipret)

The news correspondents report that additional information may be obtained from M. Mallaney, Genentech Inc, Late Stage Pharmaceut Dev, San Francisco, CA 94080, United States. Additional authors for this research include S.H. Wang and A. Sreedhara (see also Immunology).

Keywords for this news article include: Antibodies, California, Immunology, San Francisco, United States, Blood Proteins, Immunoglobulins, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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