New Streptolysins Study Findings Recently Were Reported by Researchers at Department of Biotechnology (Molecular modeling on streptolysin-O of multidrug resistant Streptococcus pyogenes and computer aided screening and in vitro assay for novel ...)
By a News Reporter-Staff News Editor at Biotech Week -- Researchers detail new data in Biological Factors. According to news reporting out of Bangalore, India, by NewsRx editors, research stated, "Streptococcus pyogenes is a notorious pathogenic bacterium which causes various human diseases ranging from localized infections to life threatening invasive diseases. Streptolysin-O (SLO), pore-forming thiol-activated cytolysin, is the major virulent factor for streptococcal infections."
Our news journalists obtained a quote from the research from the Department of Biotechnology, "Present therapies against streptococcal infections are limited as most of the strains have developed multi-drug resistance to present generation of drugs. Hence, there is a need for alternative therapeutic substances. Structure based virtual screening is a novel platform to select lead molecules with better pharmacokinetic properties. The 3D structure of SLO (not available in native form), essential for such studies, was computationally generated and this homology model was used as probable drug target. Based on literature survey, several phytoligands from 25 medicinal plants were selected. Out of these, leads from 11 plants showed better pharmacokinetic properties. The best lead molecules were screened based on computer aided drug likeness and pharmacokinetic predictions. The inhibitory properties of selected herbal leads against SLO were studied by molecular docking. An in vitro assay was further carried out and variations observed were found to be significant (p Streptococcus pyogenes with conventional drugs. The clinical strain showed multi-drug resistance to conventional drugs. Our study revealed that numerous phytoligands have better inhibitory properties towards the toxin. We noticed that incorporation of selected herbal extracts in blood agar medium showed significant reduction in hemolysis (MIC 300?l/plate), indicating inhibition of SLO. Furthermore, the butanol extracts of selected herbal preparation based on computer aided screening showed significant inhibitory properties at 250 mcg/disc concentration. We also noticed that selected herbal formulations have better antimicrobial properties at MIC range of 300-400?l."
According to the news editors, the research concluded: "Hence, our study suggests that these herbal extracts have better inhibitory properties against the toxin as well as drug resistant Streptococcus pyogenes."
For more information on this research see: Molecular modeling on streptolysin-O of multidrug resistant Streptococcus pyogenes and computer aided screening and in vitro assay for novel herbal inhibitors. Current Computer-aided Drug Design, 2014;10(1):59-74 (see also Biological Factors).
Our news journalists report that additional information may be obtained by contacting S. Skariyachan, R & D Centre, Dept. of Biotechnology, Dayananda Sagar College of Engineering, Bangalore, 560 078, Karnataka, India. Additional authors for this research include N.S. Narayan, T.S. Aggimath, S. Nagaraj, M.S. Reddy and R. Narayanappa.
Keywords for this news article include: Asia, Pharmaceuticals, India, Drugs, Therapy, Bangalore, Exotoxins, Streptolysins, Bacterial Toxins, Pharmacokinetics, Biological Factors, Pore Forming Cytotoxic Proteins.
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