New Stem Cells Study Findings Reported from Institute for Bioengineering (Polysulfone Membranes Coated with Polymerized 3,4-Dihydroxy-L-phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human ...)
By a News Reporter-Staff News Editor at Stem Cell Week -- Investigators publish new report on Stem Cell Research. According to news reporting out of Singapore, Singapore, by NewsRx editors, research stated, "Clinical and industrial applications of human pluripotent stem cells (hPSC) require large amounts of cells that have been expanded under defined conditions. Labor-intensive techniques and ill-defined or expensive compounds and substrates are not applicable."
Our news journalists obtained a quote from the research from Institute for Bioengineering, "Here we describe a chemically defined synthetic substrate consisting of polysulfone (PSF) membranes coated with polymerized 3,4-dihydroxy-L-phenylalanine (DOPA). DOPA/PSF is inexpensive and can be easily produced at various shapes and sizes. DOPA/PSF supports long-term self-renewal of undifferentiated human embryonic (hESC) and human induced pluripotent stem cells (hiPSC) under defined conditions. Pluripotency is maintained for at least 10 passages. Adhesion of hPSC to DOPA/PSF is mainly mediated by a specific integrin heterodimer. Proliferation and gene expression patterns on DOPA/PSF and control substrates are comparable. Labor-intensive cultivation methods and use of serum or coating with proteins are not required."
According to the news editors, the research concluded: "Together, these features make DOPA/PSF attractive for applications where large-scale expansion of human pluripotent stem cells under defined conditions is essential."
For more information on this research see: Polysulfone Membranes Coated with Polymerized 3,4-Dihydroxy-L-phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human Pluripotent Stem Cells. Biomacromolecules, 2014;15(6):2067-2078. Biomacromolecules can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Biomacromolecules - www.pubs.acs.org/journal/bomaf6)
Our news journalists report that additional information may be obtained by contacting K. Kandasamy, Inst Bioengn & Nanotechnol, Singapore 138669, Singapore. Additional authors for this research include K. Narayanan, M. Ni, C. Du, A.C.A. Wan and D. Zink (see also Stem Cell Research).
Keywords for this news article include: Asia, Phenylalanine, Stem Cell Research, Aromatic Amino Acids, Essential Amino Acids
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