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New Purification Technology Study Findings Have Been Reported by Investigators at Prince Songkla University (Use of the combined phase partitioning...

July 22, 2014



New Purification Technology Study Findings Have Been Reported by Investigators at Prince Songkla University (Use of the combined phase partitioning systems for recovery of proteases from hepatopancreas of Pacific white shrimp)

By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Purification Technology. According to news reporting originating from Songkhla, Thailand, by NewsRx correspondents, research stated, "Recovery of proteases from hepatopancreas of Pacific white shrimp using three-phase partitioning (TPP) system in combination with aqueous two-phase system (ATPS) was investigated. TPP was performed using t-butanol with different crude protease extract (CPE)/t-butanol ratios."

Our news editors obtained a quote from the research from Prince Songkla University, "The interphase of system comprising CPE and t-butanol with a ratio of 1:1 in the presence of 30% ammonium sulphate yielded the highest purification fold (PF) (2.6-fold) with the recovery of 76.0%. Subsequently, TPP fraction was subjected to ATPS. Effects of phase compositions including PEG molecular weight (MW) and concentration as well as types and concentration of salts on partitioning of proteases were studied. ATPS comprising PEG1000 (15%, w/w) and magnesium sulphate (25%, w/w) provided the best condition for the maximal partitioning of proteases into the top phase and gave the highest PF (8.6-fold). The yield of 65.5% was obtained. ATPS fraction was further mixed with PEG8000 and several salts for back extraction (BE). BE using 25% PEG8000 and 10% MgSO4 gave the highest PF (9.9-fold) with the yield of 46.2%. Based on electrophoresis and activity staining, the fractionated proteases had the MW of 36 and 26 kDa."

According to the news editors, the research concluded: "Therefore, the combined partitioning systems, TPP-ATPS-BE, could be effectively used to recover and purify proteases from hepatopancreas of Pacific white shrimp."

For more information on this research see: Use of the combined phase partitioning systems for recovery of proteases from hepatopancreas of Pacific white shrimp. Separation and Purification Technology, 2014;129():57-63. Separation and Purification Technology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Separation and Purification Technology - www.elsevier.com/wps/product/cws_home/600251)

The news editors report that additional information may be obtained by contacting T. Senphan, Prince Songkla University, Fac Agroind, Dept. of Food Technol, Hat Yai 90112, Songkhla, Thailand (see also Purification Technology).

Keywords for this news article include: Asia, Songkhla, Thailand, Protease, Enzymes and Coenzymes, Purification Technology

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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