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New Findings from Eli Lilly in Neutral Amino Acids Provides New Insights (Control of galactosylated glycoforms distribution in cell culture system)

July 23, 2014



By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Neutral Amino Acids. According to news originating from Indianapolis, Indiana, by NewsRx correspondents, research stated, "Cell culture process conditions including media components and bioreactor operation conditions have a profound impact on recombinant protein quality attributes. Considerable changes in the distribution of galactosylated glycoforms (G0F, G1F, and G2F) were observed across multiple CHO derived recombinant proteins in development at Eli Lilly and Company when switching to a new chemically defined (CD) media platform condition."

Our news journalists obtained a quote from the research from Eli Lilly, "In the new CD platform, significantly lower G0F percentages and higher G1F and G2F were observed. These changes were of interest as glycosylation heterogeneity can impact the effectiveness of a protein. A systematic investigation was done to understand the root cause of the change and control strategy for galactosylated glycoforms distribution. It was found that changes in asparagine concentration could result in a corresponding change in G0F, G1F, and G2F distribution. A follow-up study examined a wider range of asparagine concentration and it was found that G0F, G1F, and G2F percentage could be titrated by adjusting asparagine concentration. The observed changes in heterogeneity from changing asparagine concentration are due to resulting changes in ammonium metabolism. Further study ascertained that different integrated ammonium level during the cell culture process could control G0F, G1F, and G2F percentage distribution."

According to the news editors, the research concluded: "A mechanism hypothesis is proposed that integrated ammonium level impacts intracellular pH, which further regulates -1, 4 galactosyltransferase activity."

For more information on this research see: Control of galactosylated glycoforms distribution in cell culture system. Biotechnology Progress, 2014;30(3):547-553. Biotechnology Progress can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (American Chemical Society - www.acs.org; Biotechnology Progress - www.pubs.acs.org/journal/bipret)

The news correspondents report that additional information may be obtained from N.A. McCracken, Eli Lilly & Co, Lilly Corp Center, Bioprod Res & Dev, Indianapolis, IN 46285, United States. Additional authors for this research include R. Kowle and A. Ouyang (see also Neutral Amino Acids).

Keywords for this news article include: Asparagine, Indianapolis, United States, Basic Amino Acids, Diamino Amino Acids, Neutral Amino Acids, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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