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New Escherichia coli Infections Study Findings Have Been Reported by P. Saccardo and Co-Researchers (Effect of the DnaK chaperone on the...

July 23, 2014



New Escherichia coli Infections Study Findings Have Been Reported by P. Saccardo and Co-Researchers (Effect of the DnaK chaperone on the conformational quality of JCV VP1 virus-like particles produced in Escherichia coli)

By a News Reporter-Staff News Editor at Biotech Week -- Investigators discuss new findings in Gram-Negative Bacterial Infections. According to news reporting from Barcelona, Spain, by NewsRx journalists, research stated, "Protein nanoparticles such as virus-like particles (VLPs) can be obtained by recombinant protein production of viral capsid proteins and spontaneous self-assembling in cell factories. Contrarily to infective viral particles, VLPs lack infective viral genome while retaining important viral properties like cellular tropism and intracellular delivery of internalized molecules."

The news correspondents obtained a quote from the research, "These properties make VLPs promising and fully biocompatible nanovehicles for drug delivery. VLPs of human JC virus (hJCV) VP1 capsid protein produced in Escherichia coli elicit variable hemagglutination properties when incubated at different NaCl concentrations and pH conditions, being optimal at 200 mM NaCl and at pH range between 5.8 and 7.5. In addition, the presence or absence of chaperone DnaK in E. coli cells influence the solubility of recombinant VP1 and the conformational quality of this protein in the VLPs. The hemagglutination ability of hJCV VP1 VLPs contained in E. coli cell extracts can be modulated by buffer composition in the hemagglutination assay. It has been also determined that the production of recombinant hJCV VP1 in E. coli is favored by the absence of chaperone DnaK as observed by Western Blot analysis in different E. coli genetic backgrounds, indicating a proteolysis targeting role for DnaK. However, solubility is highly compromised in a DnaK- E. coli strain suggesting an important role of this chaperone in reduction of protein aggregates."

According to the news reporters, the research concluded: "Finally, hemagglutination efficiency of recombinant VP1 is directly related to the presence of DnaK in the producing cells."

For more information on this research see: Effect of the DnaK chaperone on the conformational quality of JCV VP1 virus-like particles produced in Escherichia coli. Biotechnology Progress, 2014;30(3):744-748. Biotechnology Progress can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (American Chemical Society - www.acs.org; Biotechnology Progress - www.pubs.acs.org/journal/bipret)

Our news journalists report that additional information may be obtained by contacting P. Saccardo, CIBER BBN, Barcelona 08193, Spain. Additional authors for this research include E. Rodriguez-Carmona, A. Villaverde and N. Ferrer-Miralles (see also Gram-Negative Bacterial Infections).

Keywords for this news article include: Spain, Viral, Virus, Europe, Virion, Genetics, Barcelona, Nucleocapsid, Gammaproteobacteria, Escherichia coli Infections, Enterobacteriaceae Infections, Gram-Negative Bacterial Infections

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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