Investigators from Xiamen University Zero in on Streptomyces (Direct proteomic mapping of Streptomyces roseosporus NRRL 11379 with precursor and insights into daptomycin biosynthesis)
By a News Reporter-Staff News Editor at Biotech Week -- Research findings on Gram-Positive Bacteria are discussed in a new report. According to news originating from Xiamen, People's Republic of China, by NewsRx correspondents, research stated, "This first-attempt study provided liquid chromatography tandem mass (LC-MS/MS) proteomics approach to explore precursor effects on daptomycin synthesis from Streptomyces roseosporus NRRL 11379. Among all, 357 and 691 differential proteins from 601 proteins in precursor group (144 h+) and 935 proteins in non-precursor group (144 h-) were identified, respectively."
Our news journalists obtained a quote from the research from Xiamen University, "Through the simulation of the 2D-protein mapping, most proteins were found in isoelectric points ranged of 4.5-10.0 as well as Mws ranged 10-100 kDa. As a result, LC-MS/MS analysis was consistence with the analytical results of two-dimensional electrophoresis (2DE) but provided much intact profiles of proteins by precursor effect on S. roseosporus. To have more insight exploration, differential proteins associated to Streptomyces spp. were defined into 14 groups of their functional classification. The major differential proteins were in transport/membrane functional group with an occupation of 12.4% for 144 h+ and 5.2% for 144 h-, respectively."
According to the news editors, the research concluded: "LC-MS/MS results as a direct proteomic mapping approach reveal more daptomycin synthetic and regulation-related proteins from precursor group in terms of methyltransferase, ATP-binding cassette (ABC) transporters, resistance proteins and regulators."
For more information on this research see: Direct proteomic mapping of Streptomyces roseosporus NRRL 11379 with precursor and insights into daptomycin biosynthesis. Journal of Bioscience and Bioengineering, 2014;117(5):591-597. Journal of Bioscience and Bioengineering can be contacted at: Soc Bioscience Bioengineering Japan, Osaka Univ, Faculty Engineering, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. (Elsevier - www.elsevier.com; Journal of Bioscience and Bioengineering - www.elsevier.com/wps/product/cws_home/505516)
The news correspondents report that additional information may be obtained from C.M. Ye, Xiamen University, Key Lab Synthet Biotechnol Xiamen City, Xiamen 361005, People's Republic of China. Additional authors for this research include I.S. Ng, K.J. Jing and Y.H. Lu (see also Gram-Positive Bacteria).
Keywords for this news article include: Asia, Xiamen, Proteins, Daptomycin, Proteomics, Amino Acids, Lipopeptides, Actinomycetales, Cyclic Peptides, Streptomycetaceae, Gram-Positive Bacteria, People's Republic of China, Gram-Positive Endospore-Forming Rods
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