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Findings in Molecular Biology Reported from Brunel University (Purification of native surfactant protein SP-A from pooled amniotic fluid and...

July 22, 2014



Findings in Molecular Biology Reported from Brunel University (Purification of native surfactant protein SP-A from pooled amniotic fluid and bronchoalveolar lavage)

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Life Science Research have been published. According to news reporting from London, United Kingdom, by NewsRx journalists, research stated, "Surfactant protein SP-A is a hydrophilic glycoprotein, similar to SP-D, which plays an important role in pulmonary surfactant homeostasis and innate immunity. SP-A is actively expressed in the alveolar type II cells and Clara cells."

The news correspondents obtained a quote from the research from Brunel University, "Their basic structure consists of triple-helical collagen region and a C-terminal carbohydrate recognition domain (CRD). By binding to the infectious microbes, SP-A (like SP-D) are involved in pathogen opsonization and agglutination and subsequent clearance of the microorganism, via recruitment of phagocytic cells via receptors for the collagen region. SP-A has also been localized at extra-pulmonary sites such as salivary epithelium, amniotic fluid, prostate glands, and semen. The presence of SP-A in fetal and maternal tissue and amniotic fluid suggests it is involved in pregnancy and labor. Native SP-A can be purified from amniotic fluid and bronchiolar lavage fluid (BALF) via affinity chromatography. In addition, we also report here a procedure to express and purify a recombinant form of trimeric CRD in Escherichia coli."

According to the news reporters, the research concluded: "The availability of highly pure native SP-A and CRD region can be central to studies that examine the diverse roles that SP-A play in surfactant homeostasis, pulmonary infection and inflammation and pregnancy."

For more information on this research see: Purification of native surfactant protein SP-A from pooled amniotic fluid and bronchoalveolar lavage. Methods In Molecular Biology, 2014;1100():257-72 (see also Life Science Research).

Our news journalists report that additional information may be obtained by contacting N. Karbani, Centre for Infection, Immunity and Disease Mechanisms, Biosciences, School of Health Sciences and Social Care, Brunel University, London, UK. Additional authors for this research include E. Dodagatta-Marri, A.S. Qaseem, P. Madhukaran, P. Waters, A.G. Tsolaki, T. Madan and U. Kishore.

Keywords for this news article include: London, Europe, United Kingdom, Life Science Research.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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