Findings in C Reactive Protein Reported from National Changhua University of Education (Use of Magnetic Nanoparticles and a Microplate Reader with Fluorescence Detection to Detect C-reactive Protein)
By a News Reporter-Staff News Editor at Proteomics Weekly -- Investigators discuss new findings in C Reactive Protein. According to news reporting originating in Changhua, Taiwan, by NewsRx journalists, research stated, "Two approaches based on magnetic nanoparticles (MNPs) have been compared to analyze C-reactive protein (CRP). Both the non-eluted and eluted MNP-1 degrees Ab-CRP-2 degrees Ab/FITC bioconjugates were measured by a microplate reader with fluorescence detection."
The news reporters obtained a quote from the research from the National Changhua University of Education, "The linear ranges for the non-elution and elution methods were 10-200 and 0.1-2.0 mu g/mL, respectively. The concentration limits of detection for the non-elution and elution methods were 2.91 and 0.04 mu g/mL, respectively. The non-elution method gave better precision and recovery than the elution method, and also showed comparable results to that of ELISA assay. The non-elution method is simple and only needs minute volumes of sample and buffer. There is no need to dissociate the fluorescence probes from the bioconjugates, and the fluorescence signals can be directly measured on the MNP-1 degrees Ab-CRP-2 degrees Ab/FITC bioconjugates."
According to the news reporters, the research concluded: "Meanwhile, samples with high CRP concentrations are not necessarily to be diluted before analysis."
For more information on this research see: Use of Magnetic Nanoparticles and a Microplate Reader with Fluorescence Detection to Detect C-reactive Protein. Journal of the Chinese Chemical Society, 2014;61(2):221-226. Journal of the Chinese Chemical Society can be contacted at: Wiley-V C H Verlag Gmbh, Boschstrasse 12, D-69469 Weinheim, Germany. (Wiley-Blackwell - www.wiley.com/; Journal of the Chinese Chemical Society - onlinelibrary.wiley.com/journal/10.1002/(ISSN)2192-6549)
Our news correspondents report that additional information may be obtained by contacting J.Y. Yang, Natl Changhua Univ Educ, Dept. of Chem, Changhua 50058, Taiwan. Additional authors for this research include Y.J. Lin, M.Y. Su, W.J. Li and M.Y. Liu (see also C Reactive Protein).
Keywords for this news article include: Asia, Taiwan, Changhua, Albumins, Immunology, Proteomics, Immunoproteins, Nanotechnology, C Reactive Protein, C-Reactive Protein, Acute-Phase Proteins, Emerging Technologies, Magnetic Nanoparticles
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