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Recent Findings from M. Muthiah and Co-Authors Yields New Data on Nanoparticles

June 10, 2014

By a News Reporter-Staff News Editor at Physics Week -- Researchers detail new data in Nanoparticles. According to news reporting from Kwangju, South Korea, by VerticalNews journalists, research stated, "We have prepared polymeric micelle-encapsulating quantum dots (QDots) for delivering the optically activatable protein Killer Red (KR) as a plasmid to cancer cells. QDots absorb light at a lower wavelength and emit light at a higher wavelength in the cell cytoplasm, activating the expressed KR."

The news correspondents obtained a quote from the research, "Once activated, KR triggers the generation of reactive oxygen species (ROS). We prepared cadmium selenide (CdSe)/zinc sulphide (ZnS) QDots and evaluated their optical properties. Subsequently, we performed morphology studies, elemental analysis, thermogravimetric analysis (TGA), and measurements of particle size and surface charge of prepared QDots encapsulated in PHEA-g-PEG-bPEI (PPP-QDot). Cellular uptake of PPP-QDot and PPP-QDot/KR nanoparticles was confirmed using confocal microscopy, and the cellular toxicity and transfection efficiency associated with uptake of PPP-QDot/KR nanoparticles were analyzed. KR expression in normal cells and cancer cells was confirmed using confocal microscopy and Western blotting. Cellular morphologies before and after intracellular activation of KR were observed using phase contrast, fluorescence, and confocal microscopy. Cell fate after exposure to blue light-emitting diode lighting was determined using apoptosis staining and a cell proliferation assay, confirming a suppression in proliferation and a reduction in metabolic activity."

According to the news reporters, the research concluded: "We determined that ROS generation contributed to cellular damage after treatment with PPP-QDot/KR nanoparticles and blue light exposure."

For more information on this research see: Intracellular delivery and activation of the genetically encoded photosensitizer Killer Red by quantum dots encapsulated in polymeric micelles. Colloids and Surfaces B-Biointerfaces, 2014;116():284-294. Colloids and Surfaces B-Biointerfaces can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands.

Our news journalists report that additional information may be obtained by contacting M. Muthiah, Korea Photon Res Center, Medical Photon Res Center, Kwangju 507779, South Korea. Additional authors for this research include S.H. Park, M. Nurunnabi, J. Lee, Y.K. Lee, H. Park, B.I. Lee, J.J. Min and I.K. Park.

Keywords for this news article include: Asia, Kwangju, South Korea, Quantum Dots, Nanotechnology, Quantum Physics, Emerging Technologies

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Source: Physics Week

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