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Findings from Complutense University Broaden Understanding of Paclitaxel Therapy

June 11, 2014



By a News Reporter-Staff News Editor at Biotech Week -- Fresh data on Drugs and Therapies are presented in a new report. According to news reporting from Madrid, Spain, by NewsRx journalists, research stated, "PH and glutathion (GSH)- responsive nanogels (NGs) based on poly-N-isopropylacrilamide (NIPA), N-hydroxyethyl acrylamide (HEAA) and tert-butyl 2-acrylamidoethyl carbamate (2AAECM) were synthesized by a microemulsion polymerization method using N, N'-cystaminebisacrylamide (CBA) as a crosslinking agent and evaluated for passive targeting of paclitaxel (PTX). Physicochemical characterizations of unloaded and PTX-loaded NGs, such as particle size, morphology, encapsulation efficiency and in vitro PTX release were also assessed."

The news correspondents obtained a quote from the research from Complutense University, "Electron microscopy techniques (SEM and TEM) as well as dynamic light scattering (DLS) analysis showed nanosized spherical hydrogels. FTIR spectra confirmed the synthesis of nanogels by free radical polymerization among vinyl groups of monomers. In vitro release was analyzed by high-performance liquid chromatography (HPLC) and differences between two NG formulations were obtained. Nanogels released almost 64% of PTX after 50 h at GSH concentrations equivalent to that in the cellular cytosol, whereas less PTX was released from NGs at pH and GSH levels similar to plasma. Cellular uptake and cytotoxicity were also demonstrated by using coumarin-6 and MTT assays, respectively, for three tumor cell lines (MCF7, HeLa and T47D). Cellular uptake assays revealed rapid uptake within 2 h and intracellular accumulation of coumarin-6-loaded nanogels after 48 h incubation. KIT assays showed changes in cell viability at different concentrations of PTX formulations, as well as pure PTX (10 mu M, 20 mu M and 30 mu M). To investigate PTX effect on cell viability, changes in cell cycle were examined by flow cytometry and a G(2)/M cell arrest was demonstrated."

According to the news reporters, the research concluded: "Overall, synthesized nanogels may be used as potential carriers for hydrophobic anticancer drugs."

For more information on this research see: pH and glutathion-responsive hydrogel for localized delivery of paclitaxel. Colloids and Surfaces B-Biointerfaces, 2014;116():247-256. Colloids and Surfaces B-Biointerfaces can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands (see also Drugs and Therapies).

Our news journalists report that additional information may be obtained by contacting E. Perez, Complutense University Madrid, Fac Med, Dept. of Biochem & Mol Biol, Polymer Mat Grp Controlled Release Bioact Cpds Bi, E-28040 Madrid, Spain. Additional authors for this research include A. Fernandez, R. Olmo, J.M. Teijon and M.D. Blanco.

Keywords for this news article include: Antineoplastics, Pharmaceuticals, Spain, Madrid, Europe, Taxoids, Nanogels, Terpenes, Paclitaxel, Hydrocarbons, Cycloparaffins, Nanotechnology, Organic Chemicals, Mitotic Inhibitors, Drugs and Therapies, Emerging Technologies

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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