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Study Results from University of Jinan Broaden Understanding of Apoptosis (A novel strategy for real-time and in situ detection of cytochrome c and...

July 4, 2014



Study Results from University of Jinan Broaden Understanding of Apoptosis (A novel strategy for real-time and in situ detection of cytochrome c and caspase-9 in Hela cells during apoptosis)

By a News Reporter-Staff News Editor at Science Letter -- Data detailed on Apoptosis have been presented. According to news reporting out of Guangzhou, People's Republic of China, by NewsRx editors, research stated, "Cytochrome c (cyt c) and caspase-9 were critical biomarkers in mitochondria-mediated apoptosis. A novel electrochemical immunosensor was developed for in situ analysis of cyt c and caspase-9 in the cytosol."

Our news journalists obtained a quote from the research from the University of Jinan, "Gold nanoparticle-polydopamine (AuNP/PDA) composites were used to fabricate the interface of the sensor. The anti-cyt c or anti-caspase-9 functionalized-immunosensor provided a biomimetic interface for immunosensing of cyt c or caspase-9 in Hela cells during apoptosis. The changes in the expression level of cyt c and caspase-9 in the cytosol upon curcumin-induced apoptosis were detected by using the proposed method, and also the influence of different concentrations and incubation times of curcumin-induced Hela cells was investigated. This method achieved a linear range (0.1-100 ?M) for standard cyt c and caspase-9, with a detection limit of 0.03 0.01 ?M for standard cyt c and 0.08 0.02 ?M for standard caspase-9. Moreover, this method was used to detect cells which could detect as low as 100 cells which expressed cyt c and caspase-9, and also the results are in good agreement with standard flow cytometry analysis."

According to the news editors, the research concluded: "The developed electrochemical immunosensor offered a simple and rapid approach for sensitive evaluation of apoptosis markers with considerable specificity and reproducibility, and also the developed strategy could be of great importance in clinical diagnosis and therapeutic research."

For more information on this research see: A novel strategy for real-time and in situ detection of cytochrome c and caspase-9 in Hela cells during apoptosis. Analyst, 2014;139(10):2499-506. (Royal Society of Chemistry - www.rsc.org/; Analyst - pubs.rsc.org/en/journals/journalissues/an)

Our news journalists report that additional information may be obtained by contacting Q. Wen, Dept. of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China. Additional authors for this research include X. Zhang, J. Cai and P.H Yang (see also Apoptosis).

Keywords for this news article include: Asia, Guangzhou, Apoptosis, Chemistry, Hela Cells, Cytochromes, Hemeproteins, Electrochemical, People's Republic of China.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Science Letter


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