Studies from Washington University in the Area of Bioinformatics Described (Composition of seed sequence is a major determinant of microRNA targeting patterns)
By a News Reporter-Staff News Editor at Information Technology Newsweekly -- Data detailed on Bioinformatics have been presented. According to news reporting originating in St. Louis, Missouri, by VerticalNews editors, the research stated, "MicroRNAs (miRNAs) are small non-coding RNAs that are extensively involved in gene expression regulation. One major roadblock in functional miRNA studies is the reliable prediction of genes targeted by miRNAs, as rules defining miRNA target recognition have not been well-established to date."
The news reporters obtained a quote from the research from Washington University, "Availability of high-throughput experimental data from a recent CLASH (cross linking, ligation and sequencing of hybrids) study has presented an unprecedented opportunity to characterize miRNA target recognition patterns, which may provide guidance for improved miRNA target prediction. The CLASH data were analysed to identify distinctive sequence features that characterize canonical and non-canonical miRNA target types. Most miRNA targets were of non-canonical type, i.e. without involving perfect pairing to canonical miRNA seed region. Different miRNAs have distinct targeting patterns, and this miRNA-to-miRNA variability was associated with seed sequence composition. Specifically, seed-based canonical target recognition was dependent on the GC content of the miRNA seed. For miRNAs with low GC content of the seed region, non-canonical targeting was the dominant mechanism for target recognition."
According to the news reporters, the research concluded: "In contrast to canonical targeting, non-canonical targeting did not lead to significant target downregulation at either the RNA or protein level."
For more information on this research see: Composition of seed sequence is a major determinant of microRNA targeting patterns. Bioinformatics, 2014;30(10):1377-1383. Bioinformatics can be contacted at: Oxford Univ Press, Great Clarendon St, Oxford OX2 6DP, England. (Oxford University Press - www.oup.com/; Bioinformatics - bioinformatics.oxfordjournals.org)
Our news correspondents report that additional information may be obtained by contacting X.W. Wang, Washington University, Sch Med, Dept. of Radiat Oncol, St Louis, MO 63108, United States.
Keywords for this news article include: Missouri, St. Louis, United States, Bioinformatics, North and Central America
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