Studies from Institute for Chemistry in the Area of Electrokinetics Described (Estrogenic compounds determination in water samples by dispersive liquid-liquid microextraction and micellar electrokinetic chromatography coupled to mass ...)
By a News Reporter-Staff News Editor at Science Letter -- Investigators publish new report on Electrokinetics. According to news reporting originating in Rome, Italy, by NewsRx journalists, research stated, "In this work, a group of 12 estrogenic compounds, i.e., four natural sexual hormones (estrone, 17 beta-estradiol, 17 alpha-estradiol and estriol), an exoestrogen (17 alpha-ethynylestradiol), a synthetic stilbene (dienestrol), a mycotoxin (zearalenone) and some of their major metabolites (2-methoxyestradiol, alpha-zearalanol, beta-zearalanol, alpha-zearalenol and beta-zearalenol) have been separated and determined by micellar electrokinetic chromatography (MEKC) coupled to electrospray ion trap mass spectrometry. For this purpose, a background electrolyte containing an aqueous solution of 45 mM of perfluorooctanoic acid (PFOA) adjusted to pH 9.0 with an ammonia solution, as MS friendly surfactant, and methanol (10% (v/v)), as organic modifier, was used."
The news reporters obtained a quote from the research from Institute for Chemistry, "To further increase the sensitivity, normal stacking mode was applied by injecting the sample dissolved in an aqueous solution of 11.5 mM of ammonium PFO (APFO) at pH 9.0 containing 10% (v/v) of methanol for 25 s. Dispersive liquid-liquid microextraction, using 110 mu L of chloroform and 500 mu L, of acetonitrile as extraction and dispersion solvents, respectively, was employed to extract and preconcentrate the target analytes from different types of environmental water samples (mineral, run-off and wastewater) containing 30% (w/v) NaCl and adjusted to pH 3.0 with 1 M HCl. The limits of detection achieved were in the range 0.04-1.10 mu g/L."
According to the news reporters, the research concluded: "The whole method was validated in terms of linearity, precision, recovery and matrix effect for each type of water, showing determination coefficients higher than 0.992 for matrix-matched calibration and absolute recoveries in the range 43-91%."
For more information on this research see: Estrogenic compounds determination in water samples by dispersive liquid-liquid microextraction and micellar electrokinetic chromatography coupled to mass spectrometry. Journal of Chromatography A, 2014;1344():109-121. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.elsevier.com/wps/product/cws_home/502688)
Our news correspondents report that additional information may be obtained by contacting G. D'Orazio, Italian Natl Res Council CNR, Inst Chem Methodol, I-00015 Rome, Italy. Additional authors for this research include M. Asensio-Ramos, J. Hernandez-Borges, S. Fanali and M.A. Rodriguez-Delgado (see also Electrokinetics).
Keywords for this news article include: Rome, Italy, Europe, Nanotechnology, Electrokinetics, Emerging Technologies
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