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Recent Findings in Chromatography Described by Researchers from Amgen (Automated high-throughput protein purification using an AKTApurifier and a...

July 4, 2014



Recent Findings in Chromatography Described by Researchers from Amgen (Automated high-throughput protein purification using an AKTApurifier and a CETAC autosampler)

By a News Reporter-Staff News Editor at Science Letter -- Research findings on Science are discussed in a new report. According to news reporting originating from Thousand Oaks, California, by NewsRx correspondents, research stated, "As the pace of drug discovery accelerates there is an increased focus on screening larger numbers of protein therapeutic candidates to identify those that are functionally superior and to assess manufacturability earlier in the process. Although there have been advances toward high throughput (HT) cloning and expression, protein purification is still an area where improvements can be made to conventional techniques."

Our news editors obtained a quote from the research from Amgen, "Current methodologies for purification often involve a tradeoff between HT automation or capacity and quality. We present an AKTA combined with an autosampler, the AKTA-AS, which has the capability of purifying up to 240 samples in two chromatographic dimensions without the need for user intervention. The AKTA-AS has been shown to be reliable with sample volumes between 0.5 mL and 100 mL, and the innovative use of a uniquely configured loading valve ensures reliability by efficiently removing air from the system as well as preventing sample cross contamination. Incorporation of a sample pump flush minimizes sample loss and enables recoveries ranging from the low tens of micrograms to milligram quantities of protein. In addition, when used in an affinity capture-buffer exchange format the final samples are formulated in a buffer compatible with most assays without requirement of additional downstream processing. The system is designed to capture samples in 96-well microplate format allowing for seamless integration of downstream HT analytic processes such as microfluidic or HPLC analysis."

According to the news editors, the research concluded: "Most notably, there is minimal operator intervention to operate this system, thereby increasing efficiency, sample consistency and reducing the risk of human error."

For more information on this research see: Automated high-throughput protein purification using an AKTApurifier and a CETAC autosampler. Journal of Chromatography A, 2014;1344():23-30. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.elsevier.com/wps/product/cws_home/502688)

The news editors report that additional information may be obtained by contacting D. Yoo, Amgen Inc, Therapeut Discovery, Thousand Oaks, CA 91320, United States. Additional authors for this research include J. Provchy, C. Park, C. Schulz and K. Walker (see also Science).

Keywords for this news article include: Science, California, Thousand Oaks, United States, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Science Letter


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