Study Data from South Central University for Nationalities Update Knowledge of Glycoside Hydrolases (Rapid screening and identification of alpha-amylase inhibitors from Garcinia xanthochymus using enzyme-immobilized magnetic nanoparticles ...)
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Enzymes and Coenzymes. According to news reporting out of Wuhan, People's Republic of China, by NewsRx editors, research stated, "Alpha-Amylase inhibitors play an important role in management of diabetes and obesity. In order to rapidly discover potent alpha-amylase inhibitors from medicinal plants, a ligands-screening method based on enzyme-immobilized magnetic nanoparticles integrated with HPLC was developed."
Our news journalists obtained a quote from the research from South Central University for Nationalities, "Amine-terminated magnetic nanoparticles were prepared for the immobilization of alpha-amylase. Based on the affinity theory, the alpha-amylase-coated magnetic nanoparticles were employed to fish out the ligands from the extracts of Garcinia xanthochymus, and the elutes were examined by HPLC. As a result, three ligands were screened out. Isolation and identification were carried out subsequently. By analyzing the UV, MS and NMR spectra, they were identified as three biflavonoids including GB2a glucoside (2), GB2a (3) and fukugetin (4). The IC50 values of the three compounds were also determined."
According to the news editors, the research concluded: "The results suggest the proposed approach is efficient and accurate, and has great potential in rapid discovery of drug candidates from medical plants."
For more information on this research see: Rapid screening and identification of alpha-amylase inhibitors from Garcinia xanthochymus using enzyme-immobilized magnetic nanoparticles coupled with HPLC and MS. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2014;960():166-173. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands (see also Enzymes and Coenzymes).
Our news journalists report that additional information may be obtained by contacting Y.F. Li, South Cent Univ Nationalities, Coll Chem & Mat Sci, Wuhan 430074, People's Republic of China. Additional authors for this research include Y. Chen, C.Y. Xiao, D. Chen, Y.X. Xiao and Z.A. Mei.
Keywords for this news article include: Asia, Wuhan, Nanotechnology, alpha-Amylases, Glycoside Hydrolases, Emerging Technologies, Enzymes and Coenzymes, Magnetic Nanoparticles, People's Republic of China
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