Study Data from Osaka City University Update Knowledge of DNA Research (Long DNA passenger strand highly improves the activity of RNA/DNA hybrid siRNAs)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in DNA Research. According to news reporting from Osaka, Japan, by NewsRx journalists, research stated, "Small interfering RNAs (siRNAs) are potent tools in biomedical research, which can reduce the expression level of target proteins through RNAi pathway. They are composed of 19-25 bp double strand RNA (dsRNAs), therefore, stimulate dsRNAs dependent interferon responses in a non-specific manner."
The news correspondents obtained a quote from the research from Osaka City University, "This problem has prevented siRNAs from being applied as new therapeautic agents. In the present paper, we tried to circumvent interferon responses using RNA/DNA hetero siRNAs (HsiRNAs) composed of RNA guide and DNA passenger strands. It was previously reported that siRNAs which were partially substituted with DNA had RNAi activity and that DNA substitution often caused the activity loss. In our results, HsiRNAs, in which the passenger strand of siRNAs were exchanged with DNA also showed much lower activity than that of parental siRNAs. Here, we found that attachment of 5' flanking sequence to DNA passenger strand improved the activity of HsiRNAs. Furthermore, the effective HsiRNAs induced much lower interferon responses than parental siRNAs."
According to the news reporters, the research concluded: "Thus, HsiRNAs with 5' flanking sequence are expected to be novel siRNA drug candidates."
For more information on this research see: Long DNA passenger strand highly improves the activity of RNA/DNA hybrid siRNAs. Journal of Bioscience and Bioengineering, 2014;117(4):401-406. Journal of Bioscience and Bioengineering can be contacted at: Soc Bioscience Bioengineering Japan, Osaka Univ, Faculty Engineering, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. (Elsevier - www.elsevier.com; Journal of Bioscience and Bioengineering - www.elsevier.com/wps/product/cws_home/505516)
Our news journalists report that additional information may be obtained by contacting H. Ida, Osaka City University, Grad Sch Engn, Dept. of Bioengn, Sumiyoshi Ku, Osaka 5588585, Japan. Additional authors for this research include K. Fukuda, A. Tachibana and T. Tanabe (see also DNA Research).
Keywords for this news article include: Asia, Osaka, Japan, Cytokines, Interferons, DNA Research, Intercellular Signaling Peptides and Proteins
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