News Column

Studies from I. Molnar and Co-Researchers in the Area of Genetic Research Reported (Flow cytometric chromosome sorting from diploid progenitors of...

July 1, 2014



Studies from I. Molnar and Co-Researchers in the Area of Genetic Research Reported (Flow cytometric chromosome sorting from diploid progenitors of bread wheat, T. urartu, Ae. speltoides and Ae. tauschii)

By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Life Science Research is the subject of a report. According to news originating from Olomouc, Czech Republic, by NewsRx correspondents, research stated, "Key message Chromosomes 5A(u), 5S and 5D can be isolated from wild progenitors, providing a chromosome-based approach to develop tools for breeding and to study the genome evolution of wheat. Abstract The three subgenomes of hexaploid bread wheat originated from Triticum urartu (A(u)A(u)), from a species similar to Aegilops speltoides (SS) (progenitor of the B genome), and from Ae. tauschii (DD)."

Our news journalists obtained a quote from the research, "Earlier studies indicated the potential of chromosome genomics to assist gene transfer from wild relatives of wheat and discover novel genes for wheat improvement. This study evaluates the potential of flow cytometric chromosome sorting in the diploid progenitors of bread wheat. Flow karyotypes obtained by analysing DAPI-stained chromosomes were characterized and the contents of the chromosome peaks were determined. FISH analysis with repetitive DNA probes proved that chromosomes 5A(u), 5S and 5D could be sorted with purities of 78-90 %, while the remaining chromosomes could be sorted in groups of three. Twenty-five conserved orthologous set (COS) markers covering wheat homoeologous chromosome groups 1-7 were used for PCR with DNA amplified from flow-sorted chromosomes and genomic DNA."

According to the news editors, the research concluded: "These assays validated the cytomolecular results as follows: peak I on flow karyotypes contained chromosome groups 1, 4 and 6, peak II represented homoeologous group 5, while peak III consisted of groups 2, 3 and 7. The isolation of individual chromosomes of wild progenitors provides an attractive opportunity to investigate the structure and evolution of the polyploid genome and to deliver tools for wheat improvement."

For more information on this research see: Flow cytometric chromosome sorting from diploid progenitors of bread wheat, T. urartu, Ae. speltoides and Ae. tauschii. Theoretical and Applied Genetics, 2014;127(5):1091-1104. Theoretical and Applied Genetics can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA (see also Life Science Research).

The news correspondents report that additional information may be obtained from I. Molnar, Center Reg Hana Biotechnol & Agr Res, Inst Expt Bot, Olomouc 78371, Czech Republic. Additional authors for this research include M. Kubalakova, H. Simkova, A. Farkas, A. Cseh, M. Megyeri, J. Vrana, M. Molnar-Lang and J. Dolezel.

Keywords for this news article include: Europe, Olomouc, Czech Republic, Life Science Research

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


For more stories covering the world of technology, please see HispanicBusiness' Tech Channel



Source: Life Science Weekly


Story Tools






HispanicBusiness.com Facebook Linkedin Twitter RSS Feed Email Alerts & Newsletters