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Studies from Complutense University Have Provided New Information about Mycobacterium (High-throughput multiplex MIRU-VNTR typing of Mycobacterium...

July 4, 2014

Studies from Complutense University Have Provided New Information about Mycobacterium (High-throughput multiplex MIRU-VNTR typing of Mycobacterium bovis)

By a News Reporter-Staff News Editor at Health & Medicine Week -- Current study results on Gram-Positive Bacteria have been published. According to news originating from Madrid, Spain, by NewsRx correspondents, research stated, "Spoligotyping is the most widely used method for genotyping Mycobacterium bovis (M. bovis). However, its discriminatory power varies widely between countries."

Our news journalists obtained a quote from the research from Complutense University, "MIRU-VNTR typing could be a promising alternative, although it generally requires the time consuming and laborious simplex PCR assays using standard agarose gel electrophoresis. The accuracy of this approach depends on good standardization and a certain degree of expertise. This study presents a version of MIRU-VNTR based on three triplex PCRs with automatic allelic assignation of the products analyzed in capillary electrophoresis. The technique was prospectively applied to 44M. bovis and two Mycobacterium caprae (M. caprae) isolates, and 22 different MIRUVNTRtypes were obtained; with spoligotyping, only 14 different types were obtained. The proposal makes it possible to shorten response times, automate procedures, and increase accuracy, thus minimizing errors in assigning genotypes."

According to the news editors, the research concluded: "It would enable the switch from a standard limited method of genotyping M. bovis to a high-throughput discriminatory fingerprinting approach."

For more information on this research see: High-throughput multiplex MIRU-VNTR typing of Mycobacterium bovis. Research in Veterinary Science, 2014;96(3):422-425. Research in Veterinary Science can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier -; Research in Veterinary Science -

The news correspondents report that additional information may be obtained from Y. Navarro, Univ Complutense, Fac Vet, Dept. of Sanidad Anim, E-28040 Madrid, Spain. Additional authors for this research include M. Herranz, B. Romero, E. Bouza, L. Dominguez, L. de Juan and D. Garcia-De-Viedma (see also Gram-Positive Bacteria).

Keywords for this news article include: Spain, Madrid, Europe, Actinomycetales, Mycobacteriaceae, Gram-Positive Rods, Gram-Positive Bacteria, Gram-Positive Asporogenous Rods

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Source: Health & Medicine Week