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Studies Conducted at Yonsei University on Nanoparticles Recently Reported (Gold nanoparticles-based colorimetric assay for cathepsin B activity and...

July 1, 2014



Studies Conducted at Yonsei University on Nanoparticles Recently Reported (Gold nanoparticles-based colorimetric assay for cathepsin B activity and the efficiency of its inhibitors)

By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Nanoparticles are presented in a new report. According to news reporting originating in Seoul, South Korea, by NewsRx journalists, research stated, "Cathepsin B has been suggested to be a prognostic marker of melanoma, glioma, and a variety of cancers such as brain, breast, colon, esophageal, gastric, lung, ovarian, and thyroid cancers. Cathepsin B inhibitors have also been considered as anticancer drug candidates; hence, there has been a growing need for a probe which enables the selective and simple detection of cathepsin B and its inhibitors."

The news reporters obtained a quote from the research from Yonsei University, "For the purpose of selective assay, a cathepsin B-specific substrate, N,N'-diBoc-dityrosine-glycine-phenylalanine-3-(methylthio)propylamine (DBDY-Gly-Phe-MTPA) was synthesized in this study. Phe-MTPA, which was produced via cathepsin B-catalyzed hydrolysis of DBDY-Gly-Phe-MTPA, allowed aggregation of gold nanoparticles (AuNPs) leading to a color change from red to blue. When tested for cathepsins B, L, and S, this assay method exhibited AuNPs color change only in reaction to cathepsin B. The limits of detection for cathepsin B was 10 and 5 nM in the 1 and 2 h hydrolysis reactions, respectively. The efficiency of cathepsin B inhibitors such as leupeptin, antipain, and chymostatin was easily compared by the degree of color change. Moreover, IC50 values of leupeptin, antipain, and chymostatin were found to be 0.11, 0.48, and 1.78 ?M, respectively, which were similar to the results of previous studies."

According to the news reporters, the research concluded: "Therefore the colorimetric assay of cathepsin B and cathepsin B inhibitors using DBDY-Gly-Phe-MTPA and AuNPs allowed not only the selective but also the simple assay of cathepsin B and its inhibitors, which was possible with the naked eye."

For more information on this research see: Gold nanoparticles-based colorimetric assay for cathepsin B activity and the efficiency of its inhibitors. Analytical Chemistry, 2014;86(8):3825-33. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)

Our news correspondents report that additional information may be obtained by contacting C.J. Kim, Dept. of Chemical and Biomolecular Engineering, Yonsei University , Seoul 120-749, South Korea. Additional authors for this research include D.I. Lee, C. Kim, K. Lee, C.H. Lee and I.S Ahn (see also Nanoparticles).

Keywords for this news article include: Asia, Seoul, South Korea, Nanotechnology, Emerging Technologies.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly