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Researchers from National Institutes of Health Report Recent Findings in Biochemistry (Uniform Fluorescent Nanobioprobes for Pathogen Detection)

July 1, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Biochemistry have been published. According to news reporting from Bethesda, Maryland, by NewsRx journalists, research stated, "Manipulating biochemical reactions in living cells to synthesize nanomaterials is an attractive strategy to realize their synthesis that cannot take place in nature. Yeast cells have been skillfully utilized to produce desired nanoparticles through spatiotemporal coupling of intracellular nonrelated biochemical reaction pathways for formation of fluorescent CdSe quantum dots."

The news correspondents obtained a quote from the research from the National Institutes of Health, "Here, we have successfully transformed Staphylococcus aureus cells into cellular beacons (fluorescing cells), all of which are highly fluorescent and photostable with perfect uniformity. Importantly, on the basis of such cells, we efficiently fabricated fluorescent nanobioprobes by a specific interaction between the protein A expressed on the S. aureus surface and the Fc fragment domain of antibodies, avoiding the use of other common methods for cell surface modifications, such as molecular covalent connection or more difficult genetic and metabolic engineering. Coupled with immunomagnetic beads, the resulting fluorescent-biotargeting bifunctional cells, i.e., biotargeting cellular beacons, can be employed as nanobioprobes for detection of viruses, bacteria, and tumor cells. With this method, H9N2 AIV can be detected specifically with a limit of 8.94 ng/mL (based on protein content)."

According to the news reporters, the research concluded: "Furthermore, diverse probes for detection of different pathogens or for other biomedical applications can be easily obtained by simply changing the antibody conjugated to the cell surface."

For more information on this research see: Uniform Fluorescent Nanobioprobes for Pathogen Detection. ACS Nano, 2014;8(5):5116-5124. ACS Nano can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society -; ACS Nano -

Our news journalists report that additional information may be obtained by contacting L.H. Xiong, NICHD, Sect Mol Morphogenesis, PCRM, National Institutes of Health, Bethesda, MD 20892, United States. Additional authors for this research include R. Cui, Z.L. Zhang, X. Yu, Z.X. Xie, Y.B. Shi and D.W. Pang (see also Biochemistry).

Keywords for this news article include: Bethesda, Maryland, Biochemical, Biochemistry, United States, North and Central America

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Source: Life Science Weekly

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