News Column

Researchers at Institute of Biotechnology Target DNA Vaccines (Enhancement of DNA vaccine efficacy by intracellular targeting strategies)

July 1, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Biotechnology is now available. According to news reporting originating in Lisbon, Portugal, by NewsRx journalists, research stated, "Immune response against an encoded antigenic protein can be elicited by including targeting sequences to DNA vaccines that promote protein sorting to processing pathways, related with antigen presentation by major histocompatibility complexes (MHC). Candidate DNA vaccines coding for neuraminidase 3 of the avian influenza virus were designed to encode different sequences that direct the protein to specific cellular compartments such as endoplasmic reticulum (i.e., adenovirus E1A), lysosomes (i.e., LAMP), and the combination of protein targeting to the endoplasmic reticulum and lysosome (i.e., E1A-LAMP)."

The news reporters obtained a quote from the research from the Institute of Biotechnology, "The DNA vaccine prototypes were engineered by biomolecular techniques and subsequently produced in E. coli cells. The biological activity of the vaccines was tested firstly in vitro, in Chinese hamster ovary cells, through flow cytometry and real-time polymerase chain reaction analysis."

According to the news reporters, the research concluded: "Then, an essential in vivo study was performed in chickens, in order to evaluate the efficacy of DNA prototype vaccines, by measuring the antibody production by enzyme-linked immunosorbent assay."

For more information on this research see: Enhancement of DNA vaccine efficacy by intracellular targeting strategies. Methods In Molecular Biology, 2014;1143():33-59 (see also Biotechnology).

Our news correspondents report that additional information may be obtained by contacting E.B. Freitas, Institute of Biotechnology and Bioengineering, Centre for Chemical and Biological Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049-001, Lisbon, Portugal. Additional authors for this research include A.M. Henriques, M. Fevereiro, D.M. Prazeres and G.A Monteiro.

Keywords for this news article include: Biotechnology, Lisbon, Europe, Portugal, Viral DNA, DNA Research, DNA Vaccines, Synthetic Vaccines.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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