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Researchers at Institute for Cancer Research and Treatment (IRCCS) Have Reported New Data on Proteomics (A proteomic approach to investigate AuNPs...

July 1, 2014

Researchers at Institute for Cancer Research and Treatment (IRCCS) Have Reported New Data on Proteomics (A proteomic approach to investigate AuNPs effects in Balb/3T3 cells)

By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Proteomics is the subject of a report. According to news originating from Genoa, Italy, by NewsRx correspondents, research stated, "Although gold nanoparticles (AuNPs) are currently used in several industrial products and biomedical applications, information about their biological effects is very limited. Thus, it is becoming crucial to assess their safety and adequately investigate the complexity of cell-nanoparticles interactions."

Our news journalists obtained a quote from the research from Institute for Cancer Research and Treatment (IRCCS), "In this work, the Balb/3T3 mouse fibroblast cell line was selected as an in vitro model to study the effects of AuNPs. Alteration of cellular processes and biochemical pathways caused by AuNPs exposure was investigated by analysing the differentially expressed proteome. Of interest was the difference observed in the protein pattern expression of cells exposed to AuNPs. It was found that 88 and 83 proteins were de-regulated after exposure to 5 and 15 nm AuNPs, respectively. Analysis of the proteome revealed that AuNPs triggers several pathways related to cellular growth and proliferation, cell morphology, cell cycle regulation, cellular function and maintenance, oxidative stress, and inflammatory response. Moreover, SPR analysis showed an increase of ECM proteins biosynthesis in cells exposed to AuNPs. We observed by TEM analysis that NPs are internalized and confined mainly in autophagosomes. Endoplasmic reticulum stressed and modification at mitochondrial level occurred. This study aims to improve existing knowledge necessary for a correct assessment of the balance between AuNPs potential adverse and beneficial effects and might have important implications for biomedical applications (e.g. nanomedicine)."

According to the news editors, the research concluded: "To conclude proteomics link to system biology analysis is a valuable tool to understand and predict nanoparticles' toxicity, furthermore it has the potential to reveal pathways that may not be immediately evident with classical toxicological assays."

For more information on this research see: A proteomic approach to investigate AuNPs effects in Balb/3T3 cells. Toxicology Letters, 2014;228(2):111-126. Toxicology Letters can be contacted at: Elsevier Ireland Ltd, Elsevier House, Brookvale Plaza, East Park Shannon, Co, Clare, 00000, Ireland. (Elsevier -; Toxicology Letters -

The news correspondents report that additional information may be obtained from S. Gioria, IRCCS Azienda Osped Univ San Martino, IST Ist Nazl Ric Cancro, I-16132 Genoa, Italy. Additional authors for this research include H. Chassaigne, D. Carpi, A. Parracino, S. Meschini, P. Barboro and F. Rossi (see also Proteomics).

Keywords for this news article include: Genoa, Italy, Europe, 3T3 Cells, Cell Line, Proteomics, Fibroblasts, Nanoparticle, Nanotechnology, Emerging Technologies

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Source: Life Science Weekly

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