Research from University of Michigan Reveals New Findings on Chemical Neuroscience (In vivo calibration of microdialysis using infusion of stable-isotope labeled neurotransmitters)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Life Science Research. According to news reporting out of Ann Arbor, Michigan, by NewsRx editors, research stated, "In vivo calibration of microdialysis probes is required for interpreting measured concentrations. The most popular method of in vivo calibration is no-net-flux (NNF), which requires infusing several concentrations of neurotransmitters to determine in vivo recoveries (extraction fraction or Ed) and extracellular concentrations."
Our news journalists obtained a quote from the research from the University of Michigan, "A new method for in vivo calibration of microdialysis of neurotransmitters using glutamate (GLU) and dopamine (DA) as model analytes is reported. (13)C6-DA and (13)C5-GLU were perfused through microdialysis probes as internal calibrators. Using liquid chromatography with mass spectrometry, it was possible to distinguish the (13)C-forms from the endogenous forms of each neurotransmitter. Ed was directly calculated by measuring the loss of the (13)C-forms during infusion. The measured endogenous (12)C forms of the neurotransmitters could be corrected for Ed to give calibrated extracellular concentrations in vivo. Retrodialysis of stable-isotope-labeled (SIL) neurotransmitters gave Ed and extracellular concentrations of (13)C5-GLU and (13)C6-DA that matched no-net-flux measurements; however, the values were obtained in a fraction of time because no added measurements were required to obtain the calibration. Ed was reduced during uptake inhibition for GLU and DA when measured by SIL retrodialysis. Because Ed is directly measured at each microdialysis fraction, it was possible to monitor changes in Ed under transient conditions created by systemic injection of uptake inhibitors. The results show that DA and GLU concentrations are underestimated by as much as 50% if not corrected for Ed during uptake inhibition."
According to the news editors, the research concluded: "SIL retrodialysis provides equivalent information to NNF at much reduced time and animal use."
For more information on this research see: In vivo calibration of microdialysis using infusion of stable-isotope labeled neurotransmitters. Acs Chemical Neuroscience, 2013;4(5):729-36. (American Chemical Society - www.acs.org; Acs Chemical Neuroscience - www.pubs.acs.org/journal/acncdm)
Our news journalists report that additional information may be obtained by contacting N.D. Hershey, Dept. of Chemistry, University of Michigan, Ann Arbor, MI 48109, United States (see also Life Science Research).
Keywords for this news article include: Michigan, Ann Arbor, United States, Life Science Research, North and Central America.
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