Reports Outline Hepatitis B Virus Findings from Fourth Military Medical University (Rapid high-throughput genotyping of HBV DNA using a modified hybridization-extension technique)
By a News Reporter-Staff News Editor at Hepatitis Weekly -- Researchers detail new data in Liver Diseases and Conditions. According to news reporting from Xi'an, People's Republic of China, by NewsRx journalists, research stated, "China has the highest incidence of hepatitis B virus (HBV) infection worldwide. HBV genotypes have variable impacts on disease pathogenesis and drug tolerance."
The news correspondents obtained a quote from the research from Fourth Military Medical University, "We have developed a technically simple and accurate method for HBV genotyping that will be applicable to pre-treatment diagnosis and individualized treatment. Multiple sequence alignments of HBV genomes from GenBank were used to design primers and probes for genotyping of HBV A through H. The hybridization was carried out on nitrocellulose (NC) membranes with probes fixed in an array format, which was followed by hybrid amplification by an extension step with DNA polymerase to reinforce the double-stranded DNA hybrids on the NC membrane and subsequent visualization using an avidin-biotin system. Genotyping results were confirmed by DNA sequencing and bioinformatics analysis using the National Center for Biotechnology Information genotyping database, and compared with results from the line probe assay. The data show that multiple sequence alignment defined a 630 bp region in the HBV PreS and S regions that was suitable for genotyping. All genotyping significant single nucleotides in the region were defined. Two-hundred-and-ninety-one HBV-positive serum samples from Northwest Chinese patients were genotyped, and the genotyping rate from the new modified hybridization-extension method was 100% compared with direct sequencing. Compared with line probe assay, the newly developed method is superior, featuring reduced reaction time, lower risk of contamination, and increased accuracy for detecting single nucleotide mutation."
According to the news reporters, the research concluded: "A novel hybridization-extension method for HBV genotyping was established, which represents a new tool for accurate and rapid SNP detection that will benefit clinical testing."
For more information on this research see: Rapid high-throughput genotyping of HBV DNA using a modified hybridization-extension technique. Physical Chemistry Chemical Physics, 2013;15(41):18179-84. (Royal Society of Chemistry - www.rsc.org/; Physical Chemistry Chemical Physics - pubs.rsc.org/en/journals/journalissues/cp)
Our news journalists report that additional information may be obtained by contacting H. Bao, The State Key Laboratory of Cancer Biology, Dept. of Pharmacogenomics, School of Pharmacy, The Fourth Military Medical University, 169 Changle West Road, Xi'an 710032, People's Republic of China. Additional authors for this research include W. Zhao, B. Ruan, Q. Wang, J. Zhao, X. Lei, W. Wang, Y. Liu, J. Sun, A. Xiang, Y. Guo and Z. Yan (see also Liver Diseases and Conditions).
Keywords for this news article include: Asia, Xi'an, Genetics, Viral DNA, DNA Viruses, DNA Research, Hepatitis B Virus, Orthohepadnavirus, Digestive System Diseases, Hepadnaviridae Infections, People's Republic of China, Liver Diseases and Conditions.
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