Reports from University of Alberta Advance Knowledge in Enzymes and Coenzymes (Understanding Interactions of Functionalized Nanoparticles with Proteins: A Case Study on Lactate Dehydrogenase)
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Enzymes and Coenzymes. According to news reporting out of Edmonton, Canada, by NewsRx editors, research stated, "Nanomaterials in biological solutions are known to interact with proteins and have been documented to affect protein function, such as enzyme activity. Understanding the interactions of nanoparticles with biological components at the molecular level will allow for rational designs of nanomaterials for use in medical technologies."
Our news journalists obtained a quote from the research from the University of Alberta, "Here we present the first detailed molecular mechanics model of functionalized gold nanoparticle (NP) interacting with an enzyme (l-lactate dehydrogenase (LDH) enzyme). Molecular dynamics (MD) simulations of the response of LDH to the NP binding demonstrate that although atomic motions (dynamics) of the main chain exhibit only a minor response to the binding, the dynamics of side chains are significantly constrained in all four active sites that predict alteration in kinetic properties of the enzyme. It is also demonstrated that the 5 nm gold NPs cause a decrease in the maximal velocity of the enzyme reaction (V-max) and a trend towards a reduced affinity (increased K-m) for the -NAD binding site, while pyruvate enzyme kinetics (K-m and V-max) are not significantly altered in the presence of the gold NPs."
According to the news editors, the research concluded: "These results demonstrate that modeling of NP:protein interactions can be used to understand alterations in protein function."
For more information on this research see: Understanding Interactions of Functionalized Nanoparticles with Proteins: A Case Study on Lactate Dehydrogenase. Small, 2014;10(10):2006-2021. Small can be contacted at: Wiley-V C H Verlag Gmbh, Boschstrasse 12, D-69469 Weinheim, Germany. (Wiley-Blackwell - www.wiley.com/; Small - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1613-6829)
Our news journalists report that additional information may be obtained by contacting O. Stueker, University of Alberta, Dept. of Biol Sci, Edmonton, AB T6G 2E9, Canada. Additional authors for this research include V.A. Ortega, G.G. Goss and M. Stepanova (see also Enzymes and Coenzymes).
Keywords for this news article include: Canada, Alberta, Edmonton, Nanomaterial, Nanoparticle, Dehydrogenase, Nanotechnology, Emerging Technologies, Enzymes and Coenzymes, North and Central America
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