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Reports from L. Shi and Colleagues Advance Knowledge in Avian Influenza (Bird Flu) (Development of a DNA microarray-based multiplex assay of avian...

July 1, 2014



Reports from L. Shi and Colleagues Advance Knowledge in Avian Influenza (Bird Flu) (Development of a DNA microarray-based multiplex assay of avian influenza virus subtypes H5, H7, H9, N1, and N2)

By a News Reporter-Staff News Editor at Virus Weekly -- Investigators discuss new findings in Avian Influenza (Bird Flu). According to news reporting originating from Shenyang, People's Republic of China, by NewsRx correspondents, research stated, "Outbreaks of highly pathogenic avian influenza have caused considerable economic losses in the poultry industry and have also resulted in human deaths since 2004. Rapid subtyping of highly pathogenic avian influenza viruses(HPAIVs) in clinical specimens is a prerequisite of prompt control of disease and prevention of its spreading."

Our news editors obtained a quote from the research, "In this study, we describe development of a DNA microarray-based detection and subtyping of HPAIVs in field samples. DNA copies of matrix (M) protein genes for the H5, H7, and H9 subtypes of hemagglutinin (HA) and the N1 and N2 subtypes of neuraminidase (NA) were prepared by RT-PCR and specific primers and then spotted onto aldehyde slides to form DNA microarrays. The HPAIV samples to be tested were subjected to total RNA isolation, RT-PCR with universal primers and Cy3 labeling, and the obtained double-stranded DNAs (targets) were finally hybridized with DNA microarrays (probes). A fluorescent spot on the microarray, detected by scanning indicated positive hybridization, i.e. the involved subtype. The assay was specific as various heterologous viruses or HPAIVs of other subtypes tested were negative. No cross-hybridization among different subtypes could be detected. The assay was more sensitive than RT-PCR and chicken embryo inoculation and could be also used for field samples."

According to the news editors, the research concluded: "Summing up, the assay has proved useful for simultaneous detection and differentiation of main epidemic HPAIV subtypes."

For more information on this research see: Development of a DNA microarray-based multiplex assay of avian influenza virus subtypes H5, H7, H9, N1, and N2. Acta Virologica, 2014;58(1):14-19. Acta Virologica can be contacted at: Aepress Sro, Bajzova 7, Bratislava, 821 08, Slovakia (see also Avian Influenza (Bird Flu)).

The news editors report that additional information may be obtained by contacting L. Shi, Liaoning Prov Center Anim Dis Control & Prevent, Shenyang, People's Republic of China. Additional authors for this research include J.S. Sun, Z.P. Yang, H.M. Bao, Y.P. Jiang, Y.Z. Xiong, D. Cao, X.W. Yu, H.L. Chen, S.M. Zheng and X.R. Wang.

Keywords for this news article include: Asia, Shenyang, Genetics, DNA Research, Bird Diseases, Poultry Diseases, Influenza in Birds, RNA Virus Infections, People's Republic of China, Avian Influenza (Bird Flu), Orthomyxoviridae Infections

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Virus Weekly


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