Recent Findings from Beijing University of Chemical Technology Provides New Insights into Gene Therapy (New amphiphilic N-phosphoryl oligopeptides designed for gene delivery)
By a News Reporter-Staff News Editor at Gene Therapy Weekly -- A new study on Biotechnology is now available. According to news reporting originating from Beijing, People's Republic of China, by NewsRx correspondents, research stated, "Gene therapy is a potent tool for the treatment of cancer and other gene defect diseases, which involves using DNA that encodes a functional, therapeutic gene to replace a mutated gene. However, the DNA transfection efficiency is restricted by its negative charges and low susceptibility to endonucleases which prevent them penetrating tissue and cellular membranes."
Our news editors obtained a quote from the research from the Beijing University of Chemical Technology, "Both viral and non-viral vectors have been used for gene delivery, but the former are limited by their immunogenicity, while the latter are less efficient than their viral counterpart. Cationic amphiphilic lipopeptides whose structures can be easily modified and transformed have been used as non-viral vectors in gene delivery system due to their low cytotoxicity and high transfection efficiency. In this study, a series of cationic amphiphilic N-phosphoryl oligopeptides with varied lengths of hydrophobic tails and oligopeptide headgroups (C12-K6, C14-K6, C16-K6, Chol-K6 and C12-H6) were synthesized and used as gene delivery vectors. The affinities, abilities to condense pDNA and transfection efficiencies of the K6-lipopeptides were better than those of the H6-lipopeptides. In addition, the hydrophobic chains of the lipopeptides also affected their transfection efficiencies. The K6-lipopeptide with a hydrophobic chain of twelve carbons (C12-K6) showed the highest transfection efficiency in all these synthetic lipopeptides. At an optimal P/N ratio of 20, C12-K6 showed comparable pDNA transfection efficiency to PEI-25k, a well-defined gene delivery vector, but the cytotoxicity of C12-K6 was much lower."
According to the news editors, the research concluded: "With acceptable gene transfection efficiency and low cytotoxicity, this cationic amphiphilic lipopeptide will have promising applications in gene therapy."
For more information on this research see: New amphiphilic N-phosphoryl oligopeptides designed for gene delivery. International Journal of Pharmaceutics, 2014;468(1-2):83-90. International Journal of Pharmaceutics can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; International Journal of Pharmaceutics - www.elsevier.com/wps/product/cws_home/505513)
The news editors report that additional information may be obtained by contacting Y.F. Sun, Beijing University of Chemical Technology, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, People's Republic of China. Additional authors for this research include L. Chen, F.D. Sun, X.B. Tian and S.Z. Luo (see also Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Beijing, Gene Therapy, Lipopeptides, Oligopeptides, Bioengineering, People's Republic of China
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