News Column

New Streptomyces Study Findings Have Been Reported by Investigators at Helmholtz Institute (Unusual site-specific DNA integration into the highly...

July 3, 2014



New Streptomyces Study Findings Have Been Reported by Investigators at Helmholtz Institute (Unusual site-specific DNA integration into the highly active pseudo-attB of the Streptomyces albus J1074 genome)

By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Research findings on Gram-Positive Bacteria are discussed in a new report. According to news originating from Saarbrucken, Germany, by NewsRx correspondents, research stated, "The phi C31-encoded recombination system has become a widely used tool for genetic analysis of streptomycetes, gene therapy and generation of transgenic animals. However, the application of this system, even in the context of its natural host genus, Streptomyces, may require a specific approach for each species."

Our news journalists obtained a quote from the research from Helmholtz Institute, "In this study, we have identified a novel pseudo-attB site, called pseB4, for integration of vectors using the phi C31 system. More than 90 % of clones contained two copies of pSET152- or pOJ436-based cosmids, after their introduction into S. albus. The efficiency of the integration of phi C31-based vectors into pseB4 is therefore comparable to that of the integration into attB. Moreover, in contrast with integration into the native attB, integration into pseB4 is not polar and does not require a complementary sequence in the TT-core region."

According to the news editors, the research concluded: "Furthermore, an analysis of conjugation frequency revealed mutual inhibition of plasmid integration into either site when both the attB and pseB4 sites were present in the genome."

For more information on this research see: Unusual site-specific DNA integration into the highly active pseudo-attB of the Streptomyces albus J1074 genome. Applied Microbiology and Biotechnology, 2014;98(11):5095-5104. Applied Microbiology and Biotechnology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Applied Microbiology and Biotechnology - www.springerlink.com/content/0175-7598/)

The news correspondents report that additional information may be obtained from B. Bilyk, Helmholtz Inst Pharmaceut Res Saarland, D-66123 Saarbrucken, Germany (see also Gram-Positive Bacteria).

Keywords for this news article include: Europe, Germany, Saarbrucken, Actinomycetales, Streptomycetaceae, Gram-Positive Bacteria, Gram-Positive Endospore-Forming Rods

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


For more stories covering the world of technology, please see HispanicBusiness' Tech Channel



Source: Gene Therapy Weekly


Story Tools






HispanicBusiness.com Facebook Linkedin Twitter RSS Feed Email Alerts & Newsletters