New Findings from Zhejiang Wanli University in the Area of Essential Amino Acids Described (Folding studies of arginine kinase from Euphausia superba using denaturants)
By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Essential Amino Acids are discussed in a new report. According to news originating from Ningbo, People's Republic of China, by NewsRx correspondents, research stated, "Arginine kinase (AK) is a key metabolic enzyme for maintaining energy balance in invertebrates and studies on AK from Euphausia superba might provide important insights into the metabolic enzymes in extreme climatic marine environments. A folding study of the AK from E. superba (ESAK) has not yet been reported."
Our news journalists obtained a quote from the research from Zhejiang Wanli University, "To gain insights into the structural and folding mechanisms of ESAK, the denaturants guanidine HCl and urea were applied in this study. We purified ESAK from the muscle of E. superba and evaluated the inhibition kinetics with structural unfolding studies under various conditions. The results revealed that ESAK was almost completely inactivated when using 1.0 M guanidine HCl and 8.25 M urea. The kinetics, characterized via time-interval measurements, showed that the inactivations by guanidine HCl and urea were first-order reactions, with the kinetic processes shifting from monophases to biphases as concentrations increased. Measurements of intrinsic and ANS (anilinonaphthalene-8-sulfonate)-binding fluorescences showed that guanidine HCl and urea induced conspicuous changes in tertiary structures and followed the regular unfolding mechanisms."
According to the news editors, the research concluded: "Our study provides information regarding the folding of this muscle-derived metabolic enzyme and expands our knowledge and understanding of invertebrate metabolisms."
For more information on this research see: Folding studies of arginine kinase from Euphausia superba using denaturants. Applied Biochemistry and Biotechnology, 2014;172(8):3888-901. Applied Biochemistry and Biotechnology can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)
The news correspondents report that additional information may be obtained from Y.X. Si, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, People's Republic of China. Additional authors for this research include N.Y. Fang, W. Wang, Z.J. Wang, J.M. Yang, G.Y. Qian, S.J. Yin and Y.D Park (see also Essential Amino Acids).
The publisher's contact information for the journal Applied Biochemistry and Biotechnology is: Springer, 233 Spring Street, New York, NY 10013, USA.
Keywords for this news article include: Asia, Ningbo, Kinase, Amidines, Arginine, Guanidines, Basic Amino Acids, Diamino Amino Acids, Enzymes and Coenzymes, Essential Amino Acids, People's Republic of China.
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