New Findings from University of Calicut Update Understanding of Serine Endopeptidases (Trypsin Inhibitor from Edible Mushroom Pleurotus floridanus Active against Proteases of Microbial Origin)
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Enzymes and Coenzymes. According to news originating from Malappuram, India, by NewsRx correspondents, research stated, "Protease inhibitors can be versatile tools mainly in the fields of medicine, agriculture and food preservative applications. Fungi have been recognized as sources of protease inhibitors, although there are only few such reports on mushrooms."
Our news journalists obtained a quote from the research from the University of Calicut, "This work reports the purification and characterization of a trypsin inhibitor from the fruiting body of edible mushroom Pleurotus floridanus (PfTI) and its effect on the activity of microbial proteases. The protease inhibitor was purified up to 35-fold by DEAE-Sepharose ion exchange column, trypsin-Sepharose column and Sephadex G100 column. The isoelectric point of the inhibitor was 4.4, and its molecular mass was calculated as 37 kDa by SDS-PAGE and 38.3 kDa by MALDI-TOF. Inhibitory activity confirmation was by dot-blot analysis and zymographic activity staining. The specificity of the inhibitor toward trypsin was with Ki of 1.043 x 10(-10) M. The inhibitor was thermostable up to 90 A degrees C with maximal stability at 30 A degrees C, active over a pH range of 4-10 against proteases from Aspergillus oryzae, Bacillus licheniformis, Bacillus sp. and Bacillus amyloliquefaciens."
According to the news editors, the research concluded: "Results indicate the possibility of utilization of protease inhibitor from P. floridanus against serine proteases."
For more information on this research see: Trypsin Inhibitor from Edible Mushroom Pleurotus floridanus Active against Proteases of Microbial Origin. Applied Biochemistry and Biotechnology, 2014;173(1):167-178. Applied Biochemistry and Biotechnology can be contacted at: Humana Press Inc, 999 Riverview Drive Suite 208, Totowa, NJ 07512, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)
The news correspondents report that additional information may be obtained from P.P.M. Ali, University of Calicut, Dept. of Biotechnol, Malappuram 673635, Kerala, India. Additional authors for this research include K. Sapna, K.R.R. Mol, S.G. Bhat, M. Chandrasekaran and K.K. Elyas (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, India, Trypsin, Malappuram, Peptide Hydrolases, Enzymes and Coenzymes, Serine Endopeptidases
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