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New Enzymes and Coenzymes Study Findings Have Been Reported from University of Lucknow (Purification and characterization of a zinc-dependent...

July 1, 2014



New Enzymes and Coenzymes Study Findings Have Been Reported from University of Lucknow (Purification and characterization of a zinc-dependent cinnamyl alcohol dehydrogenase from Leucaena leucocephala, a tree legume)

By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Enzymes and Coenzymes. According to news reporting originating in Lucknow, India, by NewsRx journalists, research stated, "A cinnamyl alcohol dehydrogenase (CAD) from the secondary xylem of Leucaena leucocephala has been purified to homogeneity through successive steps of ammonium sulfate fractionation, DEAE cellulose, Sephadex G-75, and Blue Sepharose CL-6B affinity column chromatographies. CAD was purified to 514.2 folds with overall recovery of 13 % and specific activity of 812. 5 nkat/mg."

The news reporters obtained a quote from the research from the University of Lucknow, "Native and subunit molecular masses of the purified enzyme were found to be ?76 and ?38 kDa, respectively, suggesting it to be a homodimer. The enzyme exhibited highest catalytic efficiency (Kcat/Km 3.75 ?M(-1) s(-1)) with cinnamyl aldehyde among all the substrates investigated. The pH and temperature optima of the purified CAD were pH 8.8 and 40 °C, respectively. The enzyme activity was enhanced in the presence of 2.0 mM Mg(2+), while Zn(2+) at the same concentration exerted an inhibitory effect. The inclusion of 2.0 mM EDTA in the assay system activated the enzyme. The enzyme was inhibited with caffeic acid and ferulic acid in a concentration-dependent manner, while no inhibition was observed with salicylic acid."

According to the news reporters, the research concluded: "Peptide mass analysis of the purified CAD by MALDI-TOF showed a significant homology to alcohol dehydrogenases of MDR superfamily."

For more information on this research see: Purification and characterization of a zinc-dependent cinnamyl alcohol dehydrogenase from Leucaena leucocephala, a tree legume. Applied Biochemistry and Biotechnology, 2014;172(7):3414-23. Applied Biochemistry and Biotechnology can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)

Our news correspondents report that additional information may be obtained by contacting B. Pandey, Dept. of Biochemistry, University of Lucknow, Lucknow, 226007, India. Additional authors for this research include V.P. Pandey, A.K. Shasany and U.N Dwivedi (see also Enzymes and Coenzymes).

The publisher of the journal Applied Biochemistry and Biotechnology can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA.

Keywords for this news article include: Asia, India, Lucknow, Dehydrogenase, Enzymes and Coenzymes.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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