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New Cysteine Endopeptidases Findings from Catholic University Described (Effect of Cortisol on Caspases in the Co-cultured C2C12 and 3 T3-L1 Cells)

July 1, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Enzymes and Coenzymes is the subject of a report. According to news reporting originating in Taegu, South Korea, by NewsRx editors, the research stated, "The present study was carried out to understand the effect of cortisol on caspase expression in the C2C12 and 3 T3-L1 cells under co-culture system. Cells were co-cultured by using transwell inserts with a 0.4-mu m porous membrane to separate C2C12 and 3 T3-L1 preadipocyte cells."

The news reporters obtained a quote from the research from Catholic University, "Each cell type was grown independently on the transwell plates. Following cell differentiation, inserts containing 3 T3-L1 cells were transferred to C2C12 plates and inserts containing C2C12 cells were transferred to 3 T3-L1 plates. A total of 10 mu g/mu l of cortisol was added to the medium. Following treatment of cortisol for 3 days, the cells in the lower well were harvested for analysis. Caspases such as caspase 3, caspase 7, and caspase 9 were selected for the analysis. qRT-PCR results indicated the significant increase in the mRNA expression of caspase 3, caspase 7, and caspase 9. Caspase 3, 7, and 9 activities were also increased in the mono- and co-cultured C2C12 and 3 T3-L1 cells. In addition, confocal microscopical investigation indicated that cortisol increases caspase expressions in the mono- and co-cultured C2C12 and 3 T3-L1 cells."

According to the news reporters, the research concluded: "Taking all these together, we concluded that the co-culture system reflects the exact effect of cortisol on caspase expression, which is quite distinct from one dimensional mono-cultured experiments."

For more information on this research see: Effect of Cortisol on Caspases in the Co-cultured C2C12 and 3 T3-L1 Cells. Applied Biochemistry and Biotechnology, 2014;173(4):980-988. Applied Biochemistry and Biotechnology can be contacted at: Humana Press Inc, 999 Riverview Drive Suite 208, Totowa, NJ 07512, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)

Our news correspondents report that additional information may be obtained by contacting P. Muthuraman, Catholic Univ Daegu, Dept. of Food Sci & Nutr, Taegu, South Korea (see also Enzymes and Coenzymes).

Keywords for this news article include: Asia, Taegu, South Korea, Effector Caspases, Peptide Hydrolases, Enzymes and Coenzymes, Cysteine Endopeptidases, Apoptosis Regulatory Proteins

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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