Investigators from University of Sao Paulo Report New Data on Glycoside Hydrolases (A Novel beta-Glucosidase from Humicola insolens with High Potential for Untreated Waste Paper Conversion to Sugars)
By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Enzymes and Coenzymes are presented in a new report. According to news reporting out of Ribeirao Preto, Brazil, by NewsRx editors, research stated, "Humicola insolens produced a new beta-glucosidase (BglHi2) under solid-state fermentation. The purified enzyme showed apparent molecular masses of 116 kDa (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and 404 kDa (gel-filtration), suggesting that it is a homotetramer."
Our news journalists obtained a quote from the research from the University of Sao Paulo, "Mass spectrometry analysis showed amino acid sequence similarity with a beta-glucosidase from Chaetomium thermophilum. Optima of pH and temperature were 5.0 and 65 A degrees C, respectively, and the enzyme was stable for 60 min at 50 A degrees C, maintaining 71 % residual activity after 60 min at 55 A degrees C. BglHi2 hydrolyzed p-nitrophenyl-beta-d-glucopyranoside and cellobiose. Cellobiose hydrolysis occurred with high apparent affinity (K (M) = 0.24 A +/- 0.01 mmol L-1) and catalytic efficiency (k (cat)/K (M) = 1,304.92 A +/- 53.32 L mmol(-1) s(-1)). The activity was insensitive to Fe+3, Cr+2, Mn+2, Co+2, and Ni2+, and 50-60 % residual activities were retained in the presence of Pb2+, Hg2+, and Cu2+. Mixtures of pure BglHi2 or H. insolens crude extract (CE) with crude extracts from Trichoderma reesei fully hydrolyzed Whatman no. 1 paper. Mixtures of H. insolens CE with T. reesei CE or Celluclast 1.5 L fully hydrolyzed untreated printed office paper, napkin, and magazine papers after 24-48 h, and untreated cardboard was hydrolyzed by a H. insolens CE/T. reesei CE mixture with 100 % glucose yield."
According to the news editors, the research concluded: "Data revealed the good potential of BglHi2 for the hydrolysis of waste papers, promising feedstocks for cellulosic ethanol production."
For more information on this research see: A Novel beta-Glucosidase from Humicola insolens with High Potential for Untreated Waste Paper Conversion to Sugars. Applied Biochemistry and Biotechnology, 2014;173(2):391-408. Applied Biochemistry and Biotechnology can be contacted at: Humana Press Inc, 999 Riverview Drive Suite 208, Totowa, NJ 07512, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)
Our news journalists report that additional information may be obtained by contacting L.P. Meleiro, University of Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept. of Biol, BR-14040901 Ribeirao Preto, SP, Brazil. Additional authors for this research include A. Zimbardi, F.H.M. Souza, D.C. Masui, T.M. Silva, J.A. Jorge and R.P.M. Furriel (see also Enzymes and Coenzymes).
Keywords for this news article include: Brazil, Cellulases, South America, Ribeirao Preto, beta-Glucosidase, Glycoside Hydrolases, Enzymes and Coenzymes
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