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Findings in the Area of DNA Research Reported from Johns Hopkins University (Biodegradable Polymeric Nanoparticles Show High Efficacy and Specificity...

July 3, 2014



Findings in the Area of DNA Research Reported from Johns Hopkins University (Biodegradable Polymeric Nanoparticles Show High Efficacy and Specificity at DNA Delivery to Human Glioblastoma in Vitro and in Vivo)

By a News Reporter-Staff News Editor at Gene Therapy Weekly -- New research on DNA Research is the subject of a report. According to news reporting originating from Baltimore, Maryland, by NewsRx correspondents, research stated, "Current glioblastoma therapies are insufficient to prevent tumor recurrence and eventual death. Here, we describe a method to treat malignant glioma by nonviral DNA delivery using biodegradable poly(beta-amino ester)s (PBAEs), with a focus on the brain tumor initiating cells (BTICs), the tumor cell population believed to be responsible for the formation of new tumors and resistance to many conventional therapies."

Our news editors obtained a quote from the research from Johns Hopkins University, "We show transfection efficacy of >60% and low biomaterial-mediated cytotoxicity in primary human BTICs in vitro even when the BT1Cs are grown as 3-D oncospheres. Intriguingly, we find that these polymeric nanoparticles show intrinsic specificity for nonviral transfection of primary human BTICs over primary healthy human neural progenitor cells and that this specificity is not due to differences in cellular growth rate or total cellular uptake of nanoparticles. Moreover, we demonstrate that biodegradable PBAE/DNA nanoparticles can be fabricated, lyophilized, and then stored for at least 2 years without losing efficacy, increasing the translational relevance of this technology. Using lyophilized nanoparticles, we show transgene expression by tumor cells after intratumoral injection into an orthotopic murine model of human glioblastoma. PBAE/DNA nanoparticles were more effective than naked DNA at exogenous gene expression in vivo, and tumor cells were transfected more effectively than noninvaded brain parenchyma in vivo."

According to the news editors, the research concluded: "This work shows the potential of nonviral gene delivery tools to target human brain tumors."

For more information on this research see: Biodegradable Polymeric Nanoparticles Show High Efficacy and Specificity at DNA Delivery to Human Glioblastoma in Vitro and in Vivo. ACS Nano, 2014;8(5):5141-5153. ACS Nano can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Nano - www.pubs.acs.org/journal/ancac3)

The news editors report that additional information may be obtained by contacting H. Guerrero-Cazares, Johns Hopkins University, Sch Med, Dept. of Ophthalmol, Baltimore, MD 21231, United States. Additional authors for this research include S.Y. Tzeng, N.P. Young, A.O. Abutaleb, A. Quinones-Hinojosa and J.J. Green (see also DNA Research).

Keywords for this news article include: Maryland, Baltimore, DNA Research, Nanoparticle, United States, Nanotechnology, Emerging Technologies, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Gene Therapy Weekly


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