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Findings from Z.C. Luo and Co-Authors Provides New Data about Clostridium thermocellum (Cloning of LicB from Clostridium thermocellum and its...

July 1, 2014



Findings from Z.C. Luo and Co-Authors Provides New Data about Clostridium thermocellum (Cloning of LicB from Clostridium thermocellum and its efficient secretive expression of thermostable beta-1,3-1,4-glucanase)

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Gram-Positive Bacteria have been published. According to news reporting originating from Shanghai, People's Republic of China, by NewsRx correspondents, research stated, "Beta-1,3-1,4-glucanase is a widely used enzyme in brewing and in animal feed processing. To produce the bacterial enzyme at an industrial scale, the enzyme should be able to be secreted from microbial cells into fermentation broth and be stable in different conditions."

Our news editors obtained a quote from the research, "In this study, the LicB gene encoding beta-1,3-1,4-glucanase (lichenase) from Clostridium thermocellum was secretively expressed in a secretive strain, Bacillus subtilis WB800, with eight extracellular protease deletion which made LicB expressed obviously and reached 1.18 U/g cell mass. The secreted beta-1,3-1,4-glucanase was found to be active from 40 A degrees C to 80 A degrees C and achieved the optimal activity at 80 A degrees C. The enzyme also has a wide pH range (pH 4-11). The most common metal ions and chemicals were found to be inert on its activity."

According to the news editors, the research concluded: "The property of LicB-encoded beta-1,3-1,4-glucanase and its efficient secretive expression makes it a potential enzyme for industrial production and application."

For more information on this research see: Cloning of LicB from Clostridium thermocellum and its efficient secretive expression of thermostable beta-1,3-1,4-glucanase. Applied Biochemistry and Biotechnology, 2014;173(2):562-570. Applied Biochemistry and Biotechnology can be contacted at: Humana Press Inc, 999 Riverview Drive Suite 208, Totowa, NJ 07512, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)

The news editors report that additional information may be obtained by contacting Z.C. Luo, Youtell Biotech Co, Shanghai 201201, People's Republic of China. Additional authors for this research include Q.Q. Gao, X.L. Li and J. Bao (see also Gram-Positive Bacteria).

Keywords for this news article include: Asia, Shanghai, Glucanase, Enzymes and Coenzymes, Gram-Positive Bacteria, Clostridium thermocellum, People's Republic of China, Gram-Positive Endospore-Forming Rods, Gram-Positive Endospore-Forming Bacteria

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly