Findings from University of Washington in the Area of Dendritic Cell Reported (Molecular factors in dendritic cell responses to adsorbed glycoconjugates)
By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Dendritic Cell. According to news reporting originating in Seattle, Washington, by NewsRx journalists, research stated, "Carbohydrates and glycoconjugates have been shown to exert pro-inflammatory effects on the dendritic cells (DCs), supporting pathogen-induced innate immunity and antigen processing, as well as immunosuppressive effects in the tolerance to self-proteins. Additionally, the innate inflammatory response to implanted biomaterials has been hypothesized to be mediated by inflammatory cells interacting with adsorbed proteins, many of which are glycosylated."
The news reporters obtained a quote from the research from the University of Washington, "However, the molecular factors relevant for surface displayed glycoconjugate modulation of dendritic cell (DC) phenotype are unknown. Thus, in this study, a model system was developed to establish the role of glycan composition, density, and carrier cationization state on DC response. Thiol modified glycans were covalently bound to a model protein carrier, maleimide functionalized bovine serum albumin (BSA), and the number of glycans per BSA modulated. Additionally, the carrier isoelectric point was scaled from a pI of similar to 4.0 to similar to 10.0 using ethylenediamine (EDA). The DC response to the neoglycoconjugates adsorbed to wells of a 384-well plate was determined via a high throughput assay. The underlying trends in DC phenotype in relation to conjugate properties were elucidated via multivariate general linear models. It was found that glycoconjugates with more than 20 glycans per carrier had the greatest impact on the pro-inflammatory response from DCs, followed by conjugates having an isoelectric point above 9.5. Surfaces displaying terminal alpha 1-2 linked mannose structures were able to increase the inflammatory DC response to a greater extent than did any other terminal glycan structure."
According to the news reporters, the research concluded: "The results herein can be applied to inform the design of the next generation of combination products and biomaterials for use in future vaccines and implanted materials."
For more information on this research see: Molecular factors in dendritic cell responses to adsorbed glycoconjugates. Biomaterials, 2014;35(22):5862-5874. Biomaterials can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biomaterials - www.elsevier.com/wps/product/cws_home/30392)
Our news correspondents report that additional information may be obtained by contacting N.A. Hotaling, University of Washington, Dept. of Bioengn, Seattle, WA 98195, United States. Additional authors for this research include R.D. Cummings, D.M. Ratner and J.E. Babensee (see also Dendritic Cell).
Keywords for this news article include: Antigen-Presenting Cells, Seattle, Washington, Immunology, United States, Dendritic Cells, North and Central America, Mononuclear Phagocyte System
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