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Findings from University of Florida Provide New Insights into DNA Research (Multicolor Fluorescent Biosensor for Multiplexed Detection of DNA)

July 2, 2014

By a News Reporter-Staff News Editor at Biotech Week -- Data detailed on DNA Research have been presented. According to news reporting originating from Gainesville, Florida, by NewsRx correspondents, research stated, "Development of efficient methods for highly sensitive and rapid screening of specific oligonucleotide sequences is essential to the early diagnosis of serious diseases. In this work, an aggregated cationic perylene diimide (PDI) derivative was found to efficiently quench the fluorescence emission of a variety of anionic oligonucleotide-labeled fluorophores that emit at wavelengths from the visible to NIR region."

Our news editors obtained a quote from the research from the University of Florida, "This broad-spectrum quencher was then adopted to develop a multicolor biosensor via a label-free approach for multiplexed fluorescent detection of DNA. The aggregated perylene derivative exhibits a very high quenching efficiency on all ssDNA-labeled dyes associated with biosensor detection, having efficiency values of 98.3 +/- 0.9%, 97 +/- 1.1%, and 98.2 +/- 0.6% for FAM, TAMRA, and Cy5, respectively. An exonuclease-assisted autocatalytic target recycling amplification was also integrated into the sensing system. High quenching efficiency combined with autocatalytic target recycling amplification afforded the biosensor with high sensitivity toward target DNA, resulting in a detection limit of 20 pM, which is about SO-fold lower than that of traditional unamplified homogeneous fluorescent assay methods. The quencher did not interfere with the catalytic activity of nuclease, and the biosensor could be manipulated in either preaddition or postaddition manner with similar sensitivity."

According to the news editors, the research concluded: "Moreover, the proposed sensing system allows for simultaneous and multicolor analysis of several oligonucleotides in homogeneous solution, demonstrating its potential application in the rapid screening of multiple biotargets."

For more information on this research see: Multicolor Fluorescent Biosensor for Multiplexed Detection of DNA. Analytical Chemistry, 2014;86(10):5009-5016. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society -; Analytical Chemistry -

The news editors report that additional information may be obtained by contacting R. Hu, University of Florida, McKnight Brain Inst, Gainesville, FL 32611, United States. Additional authors for this research include T. Liu, X.B. Zhang, S.Y. Huan, C.C. Wu, T. Fu and W.H. Tan (see also DNA Research).

Keywords for this news article include: Florida, Biosensing, Gainesville, DNA Research, United States, Bioengineering, Bionanotechnology, Nanobiotechnology, North and Central America

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Source: Biotech Week

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