Findings from MedImmune in the Area of Immunoglobulins Described (Absolute quantitation of host cell proteins in recombinant human monoclonal antibodies with an automated CZE-ESI-MS/MS system)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Immunology. According to news reporting originating in Gaithersburg, Maryland, by NewsRx journalists, research stated, "We report the first use of CZE for absolute characterization of host cell proteins (HCPs) in recombinant human monoclonal antibodies. An electrokinetically pumped nanoelectrospray interface was used to couple CZE with a tandem mass spectrometer."
The news reporters obtained a quote from the research from MedImmune, "Three isotopic-labeled peptides (LSFDKDAMVAR, VDIVENQAMDTR, and LVSDEMVVELIEK) were synthesized by direct incorporation of an isotope-labeled lysine or arginine. The heavy-labeled peptides were spiked in the HCP digests at known concentrations. After CZE-ESI-MS/MS analysis, the peaks of native and isotopic-labeled peptides were extracted with mass tolerance 5 ppm from the electropherograms, and the ratios of peak area between native and isotopic-labeled peptides pairs were calculated. Calibration curves (the ratios of peak area versus spiked peptide amount) with R-2 values of 0.999, 0.997, and 0.999 were obtained for the three HCP peptides, and the absolute amounts of the three proteins present were determined to be at the picomole level in a 20 g sample of digested HCPs."
According to the news reporters, the research concluded: "The target proteins were present at the 7-30 ppt level in the purified HCP samples."
For more information on this research see: Absolute quantitation of host cell proteins in recombinant human monoclonal antibodies with an automated CZE-ESI-MS/MS system. Electrophoresis, 2014;35(10):1448-1452. Electrophoresis can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Electrophoresis - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1522-2683)
Our news correspondents report that additional information may be obtained by contacting G.J. Zhu, MedImmune, Dept. of Analyt Biotechnol, Gaithersburg, MD, United States. Additional authors for this research include L.L. Sun, T. Linkous, D. Kernaghan, J.B. McGivney and N.J. Dovichi (see also Immunology).
Keywords for this news article include: Maryland, Immunology, Gaithersburg, United States, Blood Proteins, Immunoglobulins, Serum Globulins, Monoclonal Antibodies, North and Central America
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