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Findings from Karolinska Institute Provides New Data about Neuroscience (Nanoscopic spine localization of Norbin, an mGluR5 accessory protein)

June 30, 2014

By a News Reporter-Staff News Editor at Pain & Central Nervous System Week -- New research on Nervous System Research is the subject of a report. According to news reporting from Solna, Sweden, by NewsRx journalists, research stated, "Norbin is a neuron-specific, cytosolic protein that interacts with the metabotropic glutamate receptor 5 (mGluR5) and has a profound impact on mGluR5 signaling. Yet, little is known about its synaptic distribution."

The news correspondents obtained a quote from the research from Karolinska Institute, "Here we have analyzed the spatial relationship between Norbin, postsynaptic density protein 95 (PSD-95), actin and mGluR5 in spines using super-resolution microscopy. Norbin was found to have a high degree of colocalization with actin and a lower degree of colocalization with PSD-95. Co-immunoprecipitation studies confirmed that interaction occurs between Norbin and actin, but not between Norbin and PSD-95. Norbin was also found to have a high degree of colocalization with the perisynaptically located mGluR5. Findings based on structured illumination microscopy (3D-SIM) of exogenous expressed Norbin-GFP were confirmed by stimulated emission depletion microscopy (STED) of immunolabeled endogenous Norbin. Norbin associates with actin rather than with PSD-95 in dendritic spines. Results regarding protein localization and colocalization performed with conventional confocal microscopy must be interpreted with great caution."

According to the news reporters, the research concluded: "The now available super-resolution microscopy techniques provide more accurate information about sub-cellular protein localization than previously was possible."

For more information on this research see: Nanoscopic spine localization of Norbin, an mGluR5 accessory protein. Bmc Neuroscience, 2014;15():45. (BioMed Central -; Bmc Neuroscience -

Our news journalists report that additional information may be obtained by contacting L. Westin, Dept. of Women's and Children's Health, Science for Life Laboratory, Karolinska Institutet, 17165 Solna, Sweden. Additional authors for this research include M. Reuss, M. Lindskog, A. Aperia and H. Brismar (see also Nervous System Research).

Keywords for this news article include: Solna, Sweden, Europe, Nervous System Research.

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Source: Pain & Central Nervous System Week

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