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Data on Oligopeptides Described by A. Garcia-Marin et al (Glutathione Immunosensing Platform Based on Total Internal Reflection Ellipsometry Enhanced...

July 1, 2014



Data on Oligopeptides Described by A. Garcia-Marin et al (Glutathione Immunosensing Platform Based on Total Internal Reflection Ellipsometry Enhanced by Functionalized Gold Nanoparticles)

By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Peptides are discussed in a new report. According to news reporting originating from Madrid, Spain, by NewsRx correspondents, research stated, "An immunosensor to detect small molecules, such as glutathione (GSH), has been developed by combination of ellipsometry and Kretschmann surface plasmon resonance (SPR). The Au thin film used for surface plasmon polariton (SPP) excitation is functionalized with anti-GSH to specifically bind GSH."

Our news editors obtained a quote from the research, "At low concentrations, the small refractive index changes caused by the low molecular weight of GSH induced only negligible shifts in the plasmon resonant energy during GSH binding. To improve sensitivity, gold nanoparticles (AuNPs) are functionalized with glutathione acting as amplifiers of the antigen antibody interaction. Changes induced by the AuNP adsorption are monitored using psi and Delta ellipsometric functions. After performing competitive assays using solutions containing different concentrations of free GSH and a constant amount of functionalized AuNPs, it was concluded that the resonant energy linearly shifts as the relative concentration of free GSH increases."

According to the news editors, the research concluded: "A detection limit for free GSH in the nanomolar range is found, demonstrating the effectiveness of AuNPs to enhance the sensitivity to immunoreactions in total internal reflection ellipsometry."

For more information on this research see: Glutathione Immunosensing Platform Based on Total Internal Reflection Ellipsometry Enhanced by Functionalized Gold Nanoparticles. Analytical Chemistry, 2014;86(10):4969-4976. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)

The news editors report that additional information may be obtained by contacting A. Garcia-Marin, Inst Madrileno Estudios Avanzados Nanociencia IMD, Madrid 28049, Spain. Additional authors for this research include J.M. Abad, E. Ruiz, E. Lorenzo, J. Piqueras and J.L. Pau (see also Peptides).

Keywords for this news article include: Spain, Madrid, Europe, Glutathione, Oligopeptides, Nanotechnology, Gold Nanoparticles, Emerging Technologies

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Source: Life Science Weekly