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Iranians develop biosensor for fetal sex determination

June 2, 2014





Science & Technology Desk

Researchers at Yazd University have developed a simple electrochemical biosensor based on amelogenin DNA by carbon paste electrode (CPE) with gold nanoparticles.

CPE is made from a mixture of conductive graphite powder and a blending liquid. The electrodes involved are assembled easily and offer a renewable surface for electron exchange.

Carbon paste electrodes belong to a special group of heterogeneous carbon electrodes. These electrodes are widely used for voltammetric measurements.

Mohammad Mazloum-Ardekani, Noushin Rajabzadeh, Ali Benvidi and Mohammad Mehdi Haydari are scientists working on the project.

The proposed biosensor in this study could be used for detection of polymerase chain reaction (PCR) in amelogenin DNA. Amelogenin is the name for a series of closely related proteins involved in amelogenesis, the development of enamel.

Differences between the X chromosome and Y chromosome versions of the amelogenin gene (AMELX and AMELY respectively) enable it to be used in sex determination of unknown human samples. AMELX's intron 1 contains a 6-bp deletion relative to intron 1 of AMELY.

This can be detected at low cost using PCR of intron 1, followed by gel electrophoresis.

CPE was modified with gold nanoparticles (AuNPs), which are helpful for immobilization of thiolated bioreceptors.

The immobilization of the probe and its hybridization with the target DNA was optimized using different experimental conditions. The modified electrode was characterized by electrochemical impedance spectroscopy and cyclic voltammetry.

The new biosensor can distinguish between complementary and non-complementary strands of amelogenin ssDNA. Genomic DNA was extracted from blood and was detected based on changes in the MB reduction signal.


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Source: Iran Daily


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