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Study Findings from University of Pardubice Broaden Understanding of Antioxidants

June 27, 2014

By a News Reporter-Staff News Editor at Science Letter -- Researchers detail new data in Antioxidants. According to news reporting originating in Pardubice, Czech Republic, by NewsRx journalists, research stated, "The following extraction techniques have been used for extracting antioxidants (apigenin, coumarin, esculetin, umbelliferone, bergapten, quercetin, rutin, scopoletin and xanthotoxin) from plant material: supercritical fluid extraction, pressurized liquid extraction, extraction by means of Soxhlet apparatus, ultrasonic extraction in ultrasonic bath, and by means of ultrasonic probe. The analytical method based on HPLC-UV detection for the determination of selected antioxidants was developed."

The news reporters obtained a quote from the research from the University of Pardubice, "For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl radical was also determined. Comparing all results the ultrasonic probe method using 0.75 g of sample extracted by 50 mL of acetonitrile in water (30%, v/v) for 25 min at room temperature and with amplitude at 60% (equal to 90 W) without pulsation was evaluated as the best tool. The most significant indicator demonstrating this statement is the antioxidant capacity expressed as gallic acid equivalent where the ultrasonic probe method showed the best results in 10 of 16 samples."

According to the news reporters, the research concluded: "Also the operability of ultrasonic probe extraction method compared to other tested methods is more favorable."

For more information on this research see: Comparison of various techniques for the extraction and determination of antioxidants in plants. Journal of Separation Science, 2014;37(7):835-44. (Wiley-Blackwell -; Journal of Separation Science -

Our news correspondents report that additional information may be obtained by contacting P. Bajerova, University of Pardubice, Faculty of Chemical Technology, Dept. of Analytical Chemistry, Pardubice, Czech Republic. Additional authors for this research include M. Adam, T. Bajer and K. Ventura (see also Antioxidants).

Keywords for this news article include: Antioxidants, Europe, Pardubice, Czech Republic, Protective Agents.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Science Letter

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