By a News Reporter-Staff News Editor at Science Letter -- Investigators publish new report on Hydrazines. According to news reporting from Dalian, People's Republic of China, by NewsRx journalists, research stated, "Hydrazide chemistry is a powerful technique in glycopeptides enrichment. However, the low density of the monolayer hydrazine groups on the conventional hydrazine-functionalized magnetic nanoparticles limits the efficiency of glycopeptides enrichment."
The news correspondents obtained a quote from the research from the Dalian University of Technology, "Herein, a novel magnetic nanoparticle grafted with poly(glycidyl methacrylate) (GMA) brushes was fabricated via reversible addition-fragmentation chain transfer (RAFT) polymerization, and a large amount of hydrazine groups were further introduced to the GMA brushes by ring-opening the epoxy groups with hydrazine hydrate. The resulting magnetic nanoparticles (denoted as Fe3O4@SiO2@GMA-NHNH2) demonstrated the high specificity of capturing glycopeptides from a tryptic digest of the sample comprising a standard non-glycosylated protein bovine serum albumin (BSA) and four standard glycoproteins with a weight ratio of 50?:?1, and the detection limit was as low as 130 fmol. In the analysis of a real complex biological sample, the tryptic digest of hepatocellular carcinoma, 179 glycosites were identified by the Fe3O4@SiO2@GMA-NHNH2 nanoparticles, surpassing that of 68 glycosites by Fe3O4@SiO2-single-NHNH2 (with monolayer hydrazine groups on the surface)."
According to the news reporters, the research concluded: "It can be expected that the magnetic nanoparticles modified with hydrazine functionalized polymer brushes via RAFT technique will improve the specificity and the binding capacity of glycopeptides from complex samples, and show great potential in the analysis of protein glycosylation in biological samples."
For more information on this research see: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst, 2014;139(9):2199-206. (Royal Society of Chemistry - www.rsc.org/; Analyst - pubs.rsc.org/en/journals/journalissues/an)
Our news journalists report that additional information may be obtained by contacting G. Huang, State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, People's Republic of China. Additional authors for this research include Z. Sun, H. Qin, L. Zhao, Z. Xiong, X. Peng, J. Ou and H. Zou (see also Hydrazines).
Keywords for this news article include: Asia, Dalian, Hydrazines, Glycopeptides, Nanotechnology, Organic Chemicals, Emerging Technologies, Magnetic Nanoparticles, People's Republic of China.
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