By a News Reporter-Staff News Editor at Science Letter -- Researchers detail new data in Molecular Research. According to news reporting from Seoul, South Korea, by NewsRx journalists, research stated, "The formation of biofilm in a membrane bioreactor depends on the production of various signaling molecules like N-acyl homoserine lactones (AHLs). In the present study, a total of 200 bacterial strains were isolated from membrane bioreactor activated sludge and screened for AHLs production using two biosensor systems, Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136."
The news correspondents obtained a quote from the research from Konkuk University, "A correlation between AHLs production and biofilm formation has been made among screened AHLs producing strains. The 16S rRNA gene sequence analysis revealed the dominance of Aeromonas and Enterobacter sp. in AHLs production; however few a species of Serratia, Leclercia, Pseudomonas, Klebsiella, Raoultella and Citrobacter were also identified. The chromatographic characterization of sludge extract showed the presence of a broad range of quorum sensing signal molecules. Further identification of sludge AHLs by thin layer chromatography bioassay and high performance liquid chromatography confirms the presence of C4-HSL, C6-HSL, C8-HSL, 3-oxo-C8-HSL, C10-HSL, C12-HSL, 3-oxo-C12-HSL and C14-HSL."
According to the news reporters, the research concluded: "The occurrence of AHLs in sludge extract and dominance of Aeromonas and Enterobacter sp. in activated sludge suggests the key role of these bacterial strains in AHLs production and thereby membrane fouling."
For more information on this research see: Isolation and Molecular Characterization of Biofouling Bacteria and Profiling of Quorum Sensing Signal Molecules from Membrane Bioreactor Activated Sludge. International Journal of Molecular Sciences, 2014;15(2):2255-2273. International Journal of Molecular Sciences can be contacted at: Mdpi Ag, Postfach, Ch-4005 Basel, Switzerland (see also Molecular Research).
Our news journalists report that additional information may be obtained by contacting H. Lade, Konkuk University, Dept. of Environm Engn, Seoul 143701, South Korea. Additional authors for this research include D. Paul and J.H. Kweon.
Keywords for this news article include: Asia, Seoul, South Korea, Molecular Research
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