News Column

Studies from University of Illinois Have Provided New Data on Sugar Alcohols

June 27, 2014



By a News Reporter-Staff News Editor at Health & Medicine Week -- A new study on Sugar Alcohols is now available. According to news reporting from Urbana, Illinois, by NewsRx journalists, research stated, "Galactitol 2-dehydrogenase (GDH) belongs to the protein subfamily of short-chain dehydrogenases/reductases and can be used to produce optically pure building blocks and for the bioconversion of bioactive compounds. An NAD(+)-dependent GDH from Rhizobium leguminosarum by. viciae 3841 (R1GDH) was cloned and overexpressed in Escherichia coil."

The news correspondents obtained a quote from the research from the University of Illinois, "The R1GDH protein was purified as an active soluble form using His-tag affinity chromatography. The molecular mass of the purified enzyme was estimated to be 28 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 114 kDa by gel filtration chromatography, suggesting that the enzyme is a homotetramer. The enzyme has an optimal pH and temperature of 9.5 and 35 degrees C, respectively. The purified recombinant RIGDH catalyzed the oxidation of a wide range of substrates, including polyvalent aliphatic alcohols and polyols, to the corresponding ketones and ketoses. Among various polyols, galactitol was the preferred substrate of RIGDH with a K-m of 8.8 mM, k(cat) of 835 min(-1) and a k(cat)/K-m of 94.9 min(-1) mM(-1)."

According to the news reporters, the research concluded: "Although GDHs have been characterized from a few other sources, RIGDH is distinguished from other GDHs by its higher specific activity for galactitol and broad substrate spectrum, making RIGDH a good choice for practical applications."

For more information on this research see: Cloning and characterization of a galactitol 2-dehydrogenase from Rhizobium legumenosarum and its application in D-tagatose production. Enzyme and Microbial Technology, 2014;58-59():44-51. Enzyme and Microbial Technology can be contacted at: Elsevier Science Inc, 360 Park Ave South, New York, NY 10010-1710, USA. (Elsevier - www.elsevier.com; Enzyme and Microbial Technology - www.elsevier.com/wps/product/cws_home/525004)

Our news journalists report that additional information may be obtained by contacting S.S. Jagtap, University of Illinois, Inst Genom Biol, Urbana, IL 61801, United States. Additional authors for this research include R. Singh, Y.C. Kang, H.M. Zhao and J.K. Lee (see also Sugar Alcohols).

Keywords for this news article include: Urbana, Cloning, Illinois, Galactitol, United States, Dehydrogenase, Sugar Alcohols, Enzymes and Coenzymes, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Health & Medicine Week


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