News Column

Studies from Department of Biochemistry and Molecular Biology Reveal New Findings on Gene Therapy

June 26, 2014

By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Current study results on Biotechnology have been published. According to news reporting originating in Bellaterra, Spain, by NewsRx journalists, research stated, "Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. In this chapter, a standard procedure for their generation and small-scale production is described."

The news reporters obtained a quote from the research from the Department of Biochemistry and Molecular Biology, "Homologous recombination in E. coli between shuttle plasmids and full-length adenovirus backbones (E1-deleted) is used for the generation of recombinant adenoviral vectors genomes. The adenovirus genomes are then analyzed to confirm their identity and integrity, and further linearized and transfected to generate a recombinant adenoviral vector in permissive human cells. These vectors are then purified by two sequential CsCl gradient centrifugations and subjected to a chromatography step in order to eliminate the CsCl and exchange buffers."

According to the news reporters, the research concluded: "Finally, the viral stock is characterized through the quantification of its viral particle content and its infectivity."

For more information on this research see: Construction, production, and purification of recombinant adenovirus vectors. Methods In Molecular Biology, 2014;1089():159-73 (see also Biotechnology).

Our news correspondents report that additional information may be obtained by contacting S. Miravet, Unitat Produccio Vectors (UPV), CBATEG, Dept. of Biochemistry and Molecular Biology, Universitat Autonoma Barcelona, Bellaterra, Spain. Additional authors for this research include M. Ontiveros, J. Piedra, C. Penalva, M. Monfar and M. Chillon.

Keywords for this news article include: Biotechnology, Spain, Europe, Bellaterra, Adenovirus, Gene Therapy, Bioengineering.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Gene Therapy Weekly

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